Evidence map›Paper›PMID 33582995›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry.

Dietmar Hammerschmid, Jeroen F van Dyck, Frank Sobott, Antonio N Calabrese

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
4.0field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Dietmar HammerschmidProtein Chemistry, Proteomics and Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp, Wilrijk, Belgium.
Jeroen F van DyckBiomolecular & Analytical Mass Spectrometry Group, Chemistry Department, University of Antwerp, Antwerp, Belgium.
Frank SobottBiomolecular & Analytical Mass Spectrometry Group, Chemistry Department, University of Antwerp, Antwerp, Belgium.
Antonio N CalabreseFaculty of Biological Sciences, School of Molecular and Cellular Biology, University of Leeds, Leeds, UK. a.calabrese@leeds.ac.uk.
University of Antwerp · BEInstitute of Structural and Molecular Biology · GBUniversity of Leeds · GB

Funding

Biotechnology and Biological Sciences Research Council BB/P000037/1
6 · The paper itself

Abstract

Native mass spectrometry and native ion mobility mass spectrometry are now established techniques in structural biology, with recent work developing these methods for the study of integral membrane proteins reconstituted in both lipid bilayer and detergent environments. Here we show how native mass spectrometry can be used to interrogate integral membrane proteins, providing insights into conformation, oligomerization, subunit composition/stoichiometry, and interactions with detergents/lipids/drugs. Furthermore, we discuss the sample requirements and experimental considerations unique to integral membrane protein native mass spectrometry research.

Indexed as

Cell MembraneDetergentsHumansIon Mobility SpectrometryLipid BilayersMembrane ProteinsProtein ConformationDetergentsLipid BilayersMembrane ProteinsDetergent micellesIon mobilityLipidsMembrane proteinsNative mass spectrometry

Identifiers

PMID33582995
OpenAlexW3133306393

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.