Evidence map›Paper›PMID 33582948›Full record

ArticleJournal of fluorescence2021

Selection and Characterization of Cell Surface Specific Aptamer and Development of Fluorescence Assay for Detection of Shigella flexneri from Water Samples.

Padma Sudharani Lavu, Bhairab Mondal, Shylaja Ramlal

Abstract read
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In one paragraph

Article in Journal of fluorescence, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Aptasensor for the Detection ofBioengineering (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Padma Sudharani LavuMicrobiology Division, Defence Food Research Laboratory, Siddharthanagar, Mysore, Karnataka, 570011, India.
Bhairab MondalMicrobiology Division, Defence Food Research Laboratory, Siddharthanagar, Mysore, Karnataka, 570011, India.
Shylaja RamlalMicrobiology Division, Defence Food Research Laboratory, Siddharthanagar, Mysore, Karnataka, 570011, India. shylaja@dfrl.drdo.in.ORCID http://orcid.org/0000-0001-6260-9522

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study demonstrates, development of ssDNA aptamers against whole cell of S. flexneri employing a whole bacterium-based Systemic Evolution of Ligands by Exponential Enrichment (SELEX). After ten rounds of SELEX, cell surface specific aptamer pool was cloned, sequenced and divided based on sequence similarities and secondary structure. Binding affinity of FITC labelled aptamer from different group were carried out by flow cytometry analysis. The dissociation constant (K

Indexed as

Aptamers, NucleotideSELEX Aptamer TechniqueShigella flexneriFluorescenceWater MicrobiologyAptamers, NucleotideAptamerFlow cytometryFluorescenceShigella flexneriWhole cell SELEX

Identifiers

PMID33582948

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.