ArticleVeterinary microbiology2021
Genomics accurately predicts antimicrobial resistance in Staphylococcus pseudintermedius collected as part of Vet-LIRN resistance monitoring.
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Article in Veterinary microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19citing papers in PubMed
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Who cites it
19 citing papers in PubMed.
- Evaluating veterinaryJournal of clinical microbiology · 2026Article
- Comparative Phenotypic and PCR-Based Methicillin Resistance Characterization of Clinical CanineAntibiotics (Basel, Switzerland) · 2026Article
- Comparative phylogenetic, antimicrobial resistance, and clinical characterization of human spondylodiscitis-associatedFrontiers in microbiology · 2026Article
- From Farm to Fork: Antimicrobial-Resistant Bacterial Pathogens in Livestock Production and the Food Chain.Veterinary sciences · 2025Review
- Antimicrobial resistance ofVeterinary world · 2025Article
- Population and pan-genomic analyses ofApplied and environmental microbiology · 2025Article
- Novel Antibacterial Approaches and Therapeutic Strategies.Antibiotics (Basel, Switzerland) · 2025Review
- Surveillance of antimicrobial resistance in hospitalized companion animals in China in 2022-23.JAC-antimicrobial resistance · 2025Article
- Comparison of commercial next-generation sequencing assays to conventional culture methods for bacterial identification and antimicrobial susceptibility of samples obtained from clinical cases of canine superficial bacterial folliculitis.Veterinary dermatology · 2025Article
- Assessment of a program for monitoring antimicrobial purchase and resistance inFrontiers in veterinary science · 2025Article
- Comparison of genotypic and phenotypic antimicrobial resistance profiles ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Whole-genome sequences ofMicrobiology resource announcements · 2024Article
- Article
- Diagnostic and commensal Staphylococcus pseudintermedius genomes reveal niche adaptation through parallel selection of defense mechanisms.Nature communications · 2023Article
- Genomic Features of Antimicrobial Resistance inInternational journal of molecular sciences · 2023Article
- Article
- Concordance between Antimicrobial Resistance Phenotype and Genotype ofAntibiotics (Basel, Switzerland) · 2022Article
- Accessory Genome Dynamics of Local and GlobalFrontiers in microbiology · 2022Article
- Absence of Host-Specific Genes in Canine and HumanAntibiotics (Basel, Switzerland) · 2021Article
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Authors and funding
90 authors.
Gregory H TysonU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States. Electronic address: Gregory.Tyson@fda.hhs.gov.
Olgica CericU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States.
Jake GuagU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States.
Sarah NemserU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States.
Stacey BorensteinU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States.
Durda SlavicUniversity of Guelph - Animal Health Laboratory, Canada.
Sarah LippertUniversity of Guelph - Animal Health Laboratory, Canada.
Rebecca McDowellUniversity of Guelph - Animal Health Laboratory, Canada.
Aparna KrishnamurthyUniversity of Guelph - Animal Health Laboratory, Canada.
Shannon KorosecManitoba Agriculture and Resource Development - Veterinary Diagnostic Services, Canada.
Cheryl FridayManitoba Agriculture and Resource Development - Veterinary Diagnostic Services, Canada.
Neil PopleManitoba Agriculture and Resource Development - Veterinary Diagnostic Services, Canada.
Matthew E SaabDiagnostic Services, Atlantic Veterinary College, University of Prince Edward Island, Canada.
Julie-Hélène FairbrotherComplexe de diagnostic et d'épidémiosurveillance vétérinaires du Québec, Canada.
Isabelle JanelleComplexe de diagnostic et d'épidémiosurveillance vétérinaires du Québec, Canada.
Deanna McMillanUniversity of Saskatchewan - Prairie Diagnostic Services Inc, Canada.
Yugendar R BommineniBronson Animal Disease Diagnostic Laboratory, United States.
David SimonBronson Animal Disease Diagnostic Laboratory, United States.
Shipra MohanBronson Animal Disease Diagnostic Laboratory, United States.
Susan SanchezAthens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, The University of Georgia, United States.
Ashley PhillipsAthens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, The University of Georgia, United States.
Paula BartlettAthens Veterinary Diagnostic Laboratory, College of Veterinary Medicine, The University of Georgia, United States.
Hemant NaikareUniversity of Georgia - Tifton Veterinary Diagnostic & Investigational Laboratory, United States.
Cynthia WatsonUniversity of Georgia - Tifton Veterinary Diagnostic & Investigational Laboratory, United States.
Orhan SahinIowa State University, United States.
Chloe StinmanIowa State University, United States.
Leyi WangUniversity of Illinois Veterinary Diagnostic Laboratory - College of Veterinary Medicine, United States.
Carol MaddoxUniversity of Illinois Veterinary Diagnostic Laboratory - College of Veterinary Medicine, United States.
Vanessa DeShamboUniversity of Illinois Veterinary Diagnostic Laboratory - College of Veterinary Medicine, United States.
Kenitra HendrixIndiana Animal Disease Diagnostic Laboratory, United States.
Debra LubelskiIndiana Animal Disease Diagnostic Laboratory, United States.
Amy BurklundKansas State University, United States.
Brian LubbersKansas State University, United States.
Debbie ReedMurray State University Breathitt Veterinary Center, United States.
Tracie JenkinsMurray State University Breathitt Veterinary Center, United States.
Erdal ErolUniversity of Kentucky, United States.
Mukeshbhai PatelUniversity of Kentucky, United States.
Stephan LockeUniversity of Kentucky, United States.
Jordan FortnerUniversity of Kentucky, United States.
Laura PeakLouisiana State University, United States.
Udeni BalasuriyaLouisiana State University, United States.
Rinosh ManiMichigan State University, United States.
Niesa KettlerMichigan State University, United States.
Karen OlsenUniversity of Minnesota Veterinary Diagnostic Lab, United States.
Shuping ZhangUniversity of Missouri Veterinary Medical Diagnostic Laboratory, United States.
Zhenyu ShenUniversity of Missouri Veterinary Medical Diagnostic Laboratory, United States.
Martha Pulido LandinezMississippi State University, Veterinary Research and Diagnostic Lab System, United States.
Jay Kay ThorntonMississippi State University, Veterinary Research and Diagnostic Lab System, United States.
Anil ThachilNorth Carolina Veterinary Diagnostic Lab System, United States.
Melissa ByrdNorth Carolina State University, United States.
Megan JacobNorth Carolina State University, United States.
Darlene KroghNorth Dakota State University Veterinary Diagnostic Laboratory, United States.
Brett WebbNorth Dakota State University Veterinary Diagnostic Laboratory, United States.
Lynn SchaanNorth Dakota State University Veterinary Diagnostic Laboratory, United States.
Amar PatilNew Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, United States.
Sarmila DasguptaNew Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, United States.
Shannon MannNew Jersey Department of Agriculture, Animal Health Diagnostic Laboratory, United States.
Laura B GoodmanCornell University, College of Veterinary Medicine, United States.
Rebecca June Franklin-GuildCornell University, College of Veterinary Medicine, United States.
Renee R AndersonCornell University, College of Veterinary Medicine, United States.
Patrick K MitchellCornell University, College of Veterinary Medicine, United States.
Brittany D CronkCornell University, College of Veterinary Medicine, United States.
Missy ApreaCornell University, College of Veterinary Medicine, United States.
Jing CuiOhio Animal Disease Diagnostic Lab, United States.
Dominika JurkovicOhio Animal Disease Diagnostic Lab, United States.
Melanie PraratOhio Animal Disease Diagnostic Lab, United States.
Yan ZhangOhio Animal Disease Diagnostic Lab, United States.
Katherine ShiplettOhio Animal Disease Diagnostic Lab, United States.
Dubra Diaz CamposDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, United States.
Joany Van Balen RubioDepartment of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, United States.
Akhilesh RamanchandranOklahoma Animal Disease Diagnostic Laboraotry, College of Veterinary Medicine, Oklahoma State University, United States.
Scott TalentOklahoma Animal Disease Diagnostic Laboraotry, College of Veterinary Medicine, Oklahoma State University, United States.
Deepanker TewariPA Veterinary Laboratory, Pennsylvania Department of Agriculture, United States.
Nagaraja ThirumalapuraPA Veterinary Laboratory, Pennsylvania Department of Agriculture, United States.
Donna KellyUniversity of Pennsylvania, New Bolton Center, United States.
Denise BarnhartUniversity of Pennsylvania, New Bolton Center, United States.
Lacey HallUniversity of Pennsylvania, New Bolton Center, United States.
Shelley RankinUniversity of Pennsylvania, Ryan Veterinary Hospital, United States.
Jaclyn DietrichUniversity of Pennsylvania, Ryan Veterinary Hospital, United States.
Stephen ColeUniversity of Pennsylvania, Ryan Veterinary Hospital, United States.
Joy ScariaAnimal Disease Research and Diagnostic Laboratory, South Dakota State University, United States.
Linto AntonyAnimal Disease Research and Diagnostic Laboratory, South Dakota State University, United States.
Sara D LawhonTexas A&M University, College of Veterinary Medicine & Biomedical Sciences, Department of Veterinary Pathobiology, United States.
Jing WuTexas A&M University, College of Veterinary Medicine & Biomedical Sciences, Department of Veterinary Pathobiology, United States.
Christine McCoyVirginia Department of Agriculture and Consumer Services- Lynchburg Regional Animal Health Laboratory, United States.
Kelly DietzVirginia Department of Agriculture and Consumer Services- Lynchburg Regional Animal Health Laboratory, United States.
Rebecca WolkingWashington State University, United States.
Trevor AlexanderWashington State University, United States.
Claire BurbickWashington State University, United States.
Renate ReimschuesselU.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, United States.
Funding
Genomic surveillance for antimicrobial resistance in animalsU18FD006716 · FDA · CORNELL UNIVERSITY · PI Laura Brunengraber Goodman · 2019 to 2026
$707kAdapting molecular food safety tests to alternative matricesU18FD005144 · FDA · CORNELL UNIVERSITY · PI GOODMAN, LAURA BRUNENGRABER · 2014 to 2019
$516kOne Health; Epidemiology of natural and deliberate contaminants (infectious and toxicities) in animals and animal foodU18FD006157 · FDA · UNIVERSITY OF GEORGIA · PI Binu Velayudhan · 2017 to 2026
$434kStudies on Antimicrobial resistance in bacteria of veterinary importanceU18FD006379 · FDA · CORNELL UNIVERSITY · PI ALTIER, CRAIG · 2018 to 2022
$300kNJDA Animal Health Diagnostic Laboratory and FDA CVM VetLIRN Program Co-operative Agreement to Safeguard Animal and Human HealthU18FD006245 · FDA · NEW JERSEY STATE DEPARTMENTR/AGRICULTURE · PI Amar Patil · 2017 to 2026
$272kHigh throughput sequencing technology in veterinary diagnostic laboratories to combat antimicrobial resistanceU18FD006861 · FDA · UNIVERSITY OF GEORGIA · PI SANCHEZ, SUSAN · 2019 to 2019
$101kOne Health; Epidemiology of natural and deliberate contaminants (infectious and tU18FD004623 · FDA · UNIVERSITY OF GEORGIA · PI SALIKI, JEREMIAH T, SANCHEZ, SUSAN · 2012 to 2016
$97kCapacity for accredited pathogen WGS in veterinary diagnosticsU18FD006448 · FDA · CORNELL UNIVERSITY · PI GOODMAN, LAURA BRUNENGRABER · 2018 to 2018
$73kExpanding the capacity to sequence bacterial genomes and perform resistome analysis to most laboratories: bridging the gap between big sequencing centers and the diagnostic laboratoryU18FD006444 · FDA · UNIVERSITY OF GEORGIA · PI SANCHEZ, SUSAN · 2018 to 2018
$63kCapacity for antibiotic susceptibility testing and bacterial sequencing at Cornell AHDCU18FD006567 · FDA · CORNELL UNIVERSITY · PI GOODMAN, LAURA BRUNENGRABER · 2018 to 2018
$24kFDA HHS U18 FD004623FDA HHS U18 FD004849FDA HHS U18 FD005144FDA HHS U18 FD005164FDA HHS U18 FD006151FDA HHS U18 FD006155FDA HHS U18 FD006156FDA HHS U18 FD006157FDA HHS U18 FD006160FDA HHS U18 FD006164FDA HHS U18 FD006165FDA HHS U18 FD006170FDA HHS U18 FD006171FDA HHS U18 FD006172FDA HHS U18 FD006173FDA HHS U18 FD006175FDA HHS U18 FD006179FDA HHS U18 FD006180FDA HHS U18 FD006181FDA HHS U18 FD006245FDA HHS U18 FD006377FDA HHS U18 FD006378FDA HHS U18 FD006379FDA HHS U18 FD006444FDA HHS U18 FD006448FDA HHS U18 FD006453FDA HHS U18 FD006460FDA HHS U18 FD006558FDA HHS U18 FD006562FDA HHS U18 FD006567FDA HHS U18 FD006593FDA HHS U18 FD006667FDA HHS U18 FD006670FDA HHS U18 FD006671FDA HHS U18 FD006673FDA HHS U18 FD006712FDA HHS U18 FD006713FDA HHS U18 FD006716FDA HHS U18 FD006861FDA HHS U18 FD006862Intramural FDA HHS FD999999
Abstract
Whole-genome sequencing (WGS) has changed our understanding of bacterial pathogens, aiding outbreak investigations and advancing our knowledge of their genetic features. However, there has been limited use of genomics to understand antimicrobial resistance of veterinary pathogens, which would help identify emerging resistance mechanisms and track their spread. The objectives of this study were to evaluate the correlation between resistance genotypes and phenotypes for Staphylococcus pseudintermedius, a major pathogen of companion animals, by comparing broth microdilution antimicrobial susceptibility testing and WGS. From 2017-2019, we conducted antimicrobial susceptibility testing and WGS on S. pseudintermedius isolates collected from dogs in the United States as a part of the Veterinary Laboratory Investigation and Response Network (Vet-LIRN) antimicrobial resistance monitoring program. Across thirteen antimicrobials in nine classes, resistance genotypes correlated with clinical resistance phenotypes 98.4 % of the time among a collection of 592 isolates. Our findings represent isolates from diverse lineages based on phylogenetic analyses, and these strong correlations are comparable to those from studies of several human pathogens such as Staphylococcus aureus and Salmonella enterica. We uncovered some important findings, including that 32.3 % of isolates had the mecA gene, which correlated with oxacillin resistance 97.0 % of the time. We also identified a novel rpoB mutation likely encoding rifampin resistance. These results show the value in using WGS to assess antimicrobial resistance in veterinary pathogens and to reveal putative new mechanisms of resistance.
Indexed as
AnimalsAnti-Bacterial AgentsBacterial ProteinsCanadaDog DiseasesDogsDrug Resistance, BacterialEpidemiological MonitoringGenomicsGenotypeMicrobial Sensitivity TestsPhenotypePhylogenyReproducibility of ResultsStaphylococcal InfectionsStaphylococcusAnti-Bacterial AgentsBacterial ProteinsMecA protein, Staphylococcus pseudintermediusAntimicrobial resistanceGenomicsStaphylococcus pseudintermedius
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