ArticlePhysiological reports2021
Overexpression of Substance P in pig airways increases MUC5AC through an NF-kβ pathway.
Article in Physiological reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Wired for immunity: neuroimmune control of the lung by sensory neurons.Nature reviews. Neuroscience · 2026Review
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- Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery.Respiratory research · 2024Review
- Role of Sensory Nerves in Pulmonary Fibrosis.International journal of molecular sciences · 2024Review
- Post COVID-19 complications and follow up biomarkers.Nanoscale advances · 2023Review
- Advancing Lung Immunology Research: An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology · 2022Article
- Identification of cholinergic cells with chemosensory traits in the porcine uterus.Cell and tissue research · 2022Article
- Analysis of Airway Vagal Neurons.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nociceptor neurons promote IgE class switch in B cells.JCI insight · 2021Article
- Gastroesophageal reflux-induced cough: the positive feedback loop of microaspiration and neurogenic inflammation.Therapeutic advances in respiratory diseaseReview
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Substance P (SP) is a tachykinin that regulates airway mucous secretion in both health and disease. Our study aimed to determine whether overexpression of SP without pre-existing inflammation was sufficient to induce changes in mucin secretion and transport in small airways. Utilizing porcine precision-cut lung slices, we measured the impact of AAV-mediated overexpression of SP on airway physiology ex vivo. Immunofluorescence signal intensity for MUC5AC was significantly increased in SP-overexpressed precision-cut lung slices compared to GFP controls. No difference in MUC5B signal intensity between treatments was detected. SP-overexpressed precision-cut lung slices also exhibited decreased IL10 mRNA, an important inhibitor of mucous cell metaplasia. Overt deficits in mucociliary transport were not noted, though a trend for decreased mean transport speed was detected in methacholine-challenged airways overexpressing SP compared to GFP controls. Pharmacologic inhibition of the NF-kβ pathway abrogated the effects of overexpression of SP on both MUC5AC and IL10. Collectively, these data suggest that overexpression of SP in the absence of existing inflammation increases MUC5AC via activation of the NF-kβ pathway. Thus, these data further highlight SP as a key driver of abnormal mucous secretion and underscore NF-kβ signaling as a pathway of potential therapeutic intervention.
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