ArticleApplied microscopy2020
Replacing critical point drying with a low-cost chemical drying provides comparable surface image quality of glandular trichomes from leaves of Millingtonia hortensis L. f. in scanning electron micrograph.
Article in Applied microscopy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
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- Going around the bend to understand the role of leg coalescence in metachronal swimming.The Journal of experimental biology · 2025Article
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- Unveiling physiological responses and modulated accumulation patterns of specialized metabolites inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- A Simple and User-Friendly Method for High-Quality Preparation of Pollen Grains for Scanning Electron Microscopy (SEM).Plants (Basel, Switzerland) · 2024Article
- Histochemical, metabolic and ultrastructural changes in leaf patelliform nectaries explain extrafloral nectar synthesis and secretion in Clerodendrum chinense.Annals of botany · 2024Article
- Chemical and structural heterogeneity of olive leaves and their trichomes.Communications biology · 2024Article
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- The use of scanning electron microscopy and fixation methods to evaluate the interaction of blood with the surfaces of medical devices.Scientific reports · 2024Article
- Evaluation of Antibacterial Activity of Selenium Nanoparticles against Food-Borne Pathogens.Microorganisms · 2023Article
- A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface.International journal of molecular sciences · 2022Article
- Cuticle Modifications and Over-Expression of the Chitin-Synthase Gene in Diflubenzuron-Resistant Phenotype.Insects · 2022Article
- Localization of the Swainsonine-Producing Chaetothyriales Symbiont in the Seed and Shoot Apical Meristem in Its HostMicroorganisms · 2022Article
- Integrative small RNA and transcriptome analysis provides insight into key role of miR408 towards drought tolerance response in cowpea.Plant cell reports · 2022Article
- Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids.Biomedicines · 2021Review
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5 authors.
Funding
Abstract
Sample preparation including dehydration and drying of samples is the most intricate part of scanning electron microscopy. Most current sample preparation protocols use critical-point drying with liquid carbon dioxide. Very few studies have reported samples that were dried using chemical reagents. In this study, we used hexamethyldisilazane, a chemical drying reagent, to prepare plant samples. As glandular trichomes are among the most fragile and sensitive surface structures found on plants, we used Millingtonia hortensis leaf samples as our study materials because they contain abundant glandular trichomes. The results obtained using this new method are identical to those produced via critical-point drying.
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