ArticleScience China. Life sciences2021
Molecular mechanism of mureidomycin biosynthesis activated by introduction of an exogenous regulatory gene ssaA into Streptomyces roseosporus.
Article in Science China. Life sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 20 citations in OpenAlex.
- Advances in tools, strategies, and applications of mining of microbial genomes for novel antimicrobials: a comprehensive review.Folia microbiologica · 2026Review
- Co-expression of a pair of interdependent regulators coding genes ovmZ and ovmW awakens the production of angucyclinones antibiotics in Streptomyces neyagawaensis.Microbial cell factories · 2024Article
- Pseudomonas aeruginosa regulator PvrA binds simultaneously to multiple pseudo-palindromic sites for efficient transcription activation.Science China. Life sciences · 2024Article
- Transcriptional regulators of secondary metabolite biosynthesis in Streptomyces.World journal of microbiology & biotechnology · 2024Review
- TheiScience · 2024Article
- Bactericidal bissulfone BScience China. Life sciences · 2024Article
- New insights into the dihydro-mureidomycin biosynthesis controlled by two unusual proteins in Streptomyces roseosporus.Microbial cell factories · 2023Article
- Quantitative characterization of filamentous fungal promoters on a single-cell resolution to discover cryptic natural products.Science China. Life sciences · 2023Article
- An intricate regulation of WblA controlling production of silent tylosin analogues and abolishment of expressible nikkomycin.Science China. Life sciences · 2023Article
- Insights into the biosynthesis of septacidin l-heptosamine moiety unveils a VOC family sugar epimerase.Acta pharmaceutica Sinica. B · 2023Article
- A visualization reporter system for characterizing antibiotic biosynthetic gene clusters expression with high-sensitivity.Communications biology · 2022Article
- SspH, a Novel HATPase Family Regulator, Controls Antibiotic Biosynthesis inAntibiotics (Basel, Switzerland) · 2022Article
- Activation of Secondary Metabolism in Red Soil-Derived Streptomycetes via Co-Culture with Mycolic Acid-Containing Bacteria.Microorganisms · 2021Article
- Activation of Cryptic Antibiotic Biosynthetic Gene Clusters Guided by RNA-seq Data from BothAntibiotics (Basel, Switzerland) · 2021Article
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8 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Mureidomycins (MRDs), a group of unique uridyl-peptide antibiotics, exhibit antibacterial activity against the highly refractory pathogen Pseudomonas aeruginosa. Our previous study showed that the cryptic MRD biosynthetic gene cluster (BGC) mrd in Streptomyces roseosporus NRRL 15998 could not be activated by its endogenous regulator 02995 but activated by an exogenous activator SsaA from sansanmycin's BGC ssa of Streptomyces sp. strain SS. Here we report the molecular mechanism for this inexplicable regulation. EMSAs and footprinting experiments revealed that SsaA could directly bind to a 14-nt palindrome sequence of 5'-CTGRCNNNNGTCAG-3' within six promoter regions of mrd. Disruption of three representative target genes (SSGG-02981, SSGG-02987 and SSGG-02994) showed that the target genes directly controlled by SsaA were essential for MRD production. The regulatory function was further investigated by replacing six regions of SSGG-02995 with those of ssaA. Surprisingly, only the replacement of 343-450 nt fragment encoding the 115-150 amino acids (AA) of SsaA could activate MRD biosynthesis. Further bioinformatics analysis showed that the 115-150 AA situated between two conserved domains of SsaA. Our findings significantly demonstrate that constitutive expression of a homologous exogenous regulatory gene is an effective strategy to awaken cryptic biosynthetic pathways in Streptomyces.
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