ArticleNature communications2021
Insertion-sequence-mediated mutations both promote and constrain evolvability during a long-term experiment with bacteria.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 1 of them a synthesis that pooled it.
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Who cites it
66 citing papers in PubMed, 1 synthesis or guideline pooled it, 129 citations in OpenAlex.
- Global prevalence of mutation in theFrontiers in microbiology · 2024Pooled it
- Genomic architecture of antimicrobial resistance and mobile genetic elements inMicrobiology spectrum · 2026Article
- Capsular Polysaccharide Safeguards a Prophage-Bacterium Symbiosis by Preventing Collateral Attack and Promoting Viral Transmission.bioRxiv : the preprint server for biology · 2026Article
- Obligate cross-feeding of metabolites is common in soil microbial communities.Nature microbiology · 2026Article
- Translation Inhibiting Antibiotics Induce an asRNA Regulating Purine Metabolism inbioRxiv : the preprint server for biology · 2026Article
- Comprehensive profiling of insertion sequences and eukaryotic-like transposons across archaeal genomes.BMC genomics · 2026Article
- Cell wall remodeling-dependent morphotype switch inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Plasmids promote antimicrobial resistance through insertion sequence-mediated gene inactivation.Nature microbiology · 2026Article
- Genomic and functional dissection of natural transformation-related genes inMicrobiology spectrum · 2026Article
- Characterizing the ecological niche of insertion sequences within prokaryotic genomes.The ISME journal · 2026Article
- Independent, ongoing clade-specific expansions of ISMicrobial genomics · 2026Article
- Genome Plasticity Depends on Positions both Inside and Outside of the Symbiosis Island of Bradyrhizobium diazoefficiens.Microbes and environments · 2026Article
- ISAntibiotics (Basel, Switzerland) · 2025Article
- Article
- Molecular characterization of colistin resistance in carbapenem-resistantMicrobiology spectrum · 2025Article
- The ISPpu9 insertion sequence of Pseudomonas putida KT2440 generates various circular intermediates enabling modular transposition.Nucleic acids research · 2025Article
- Comparative Analysis of Mammalian Adaptive Immune Loci Revealed Spectacular Divergence and Common Genetic Patterns.Molecular biology and evolution · 2025Article
- The latent cis-regulatory potential of mobile DNA in Escherichia coli.Nature communications · 2025Article
- Article
- Laboratory evolution of the bacterial genome structure through insertion sequence activation.Nucleic acids research · 2025Article
6 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insertion sequences (IS) are ubiquitous bacterial mobile genetic elements, and the mutations they cause can be deleterious, neutral, or beneficial. The long-term dynamics of IS elements and their effects on bacteria are poorly understood, including whether they are primarily genomic parasites or important drivers of adaptation by natural selection. Here, we investigate the dynamics of IS elements and their contribution to genomic evolution and fitness during a long-term experiment with Escherichia coli. IS elements account for ~35% of the mutations that reached high frequency through 50,000 generations in those populations that retained the ancestral point-mutation rate. In mutator populations, IS-mediated mutations are only half as frequent in absolute numbers. In one population, an exceptionally high ~8-fold increase in IS150 copy number is associated with the beneficial effects of early insertion mutations; however, this expansion later slowed down owing to reduced IS150 activity. This population also achieves the lowest fitness, suggesting that some avenues for further adaptation are precluded by the IS150-mediated mutations. More generally, across all populations, we find that higher IS activity becomes detrimental to adaptation over evolutionary time. Therefore, IS-mediated mutations can both promote and constrain evolvability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.