Evidence map›Paper›PMID 33579822›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

Widespread polycistronic gene expression in green algae.

Sean D Gallaher, Rory J Craig, Iniyan Ganesan, Samuel O Purvine, Sean R McCorkle, Jane Grimwood, Daniela Strenkert, Lital Davidi, Melissa S Roth, Tim L Jeffers and 6 more

Abstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

  1. The entireAntimicrobial agents and chemotherapy · 2026
    Article
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  3. Review
  4. Article
  5. Article
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  7. The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025
    Review
  8. Article
  9. Leaky ribosomal scanning enables tunable translation of bicistronic ORFs in green algae.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  10. Deciphering Transcription inbioRxiv : the preprint server for biology · 2025
    Article
  11. Article
  12. Article
  13. Article
  14. Noncanonical microprotein regulation of immunity.Molecular therapy : the journal of the American Society of Gene Therapy · 2024
    Review
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Sean D GallaherUCLA DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095; sabeeha@berkeley.edu gallaher@chem.ucla.edu.ORCID 0000-0002-9773-6051
Rory J CraigJoint Genome Institute, United States Department of Energy, Berkeley, CA 94720.ORCID 0000-0002-6262-0008
Iniyan GanesanDepartment of Plant Biology, University of California, Davis, CA 95616.ORCID 0000-0002-1082-202X
Samuel O PurvineEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354.ORCID 0000-0002-2257-2400
Sean R McCorkleBiology Department, Brookhaven National Laboratory, Upton, NY 11973.ORCID 0000-0003-1126-4071
Jane GrimwoodHudsonAlpha Genome Sequencing Center, HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806.ORCID 0000-0002-8356-8325
Daniela StrenkertDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095.ORCID 0000-0002-3420-1332
Lital DavidiDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095.ORCID 0000-0003-2698-8021
Melissa S RothDepartment of Plant and Microbial Biology, University of California, Berkeley, CA 94720.ORCID 0000-0001-7389-5982
Tim L JeffersDepartment of Plant and Microbial Biology, University of California, Berkeley, CA 94720.ORCID 0000-0002-6021-4006
Mary S LiptonEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354.
Krishna K NiyogiDepartment of Plant and Microbial Biology, University of California, Berkeley, CA 94720.ORCID 0000-0001-7229-2071
Jeremy SchmutzJoint Genome Institute, United States Department of Energy, Berkeley, CA 94720.ORCID 0000-0001-8062-9172
Steven M ThegDepartment of Plant Biology, University of California, Davis, CA 95616.ORCID 0000-0002-6397-0413
Crysten E Blaby-HaasBiology Department, Brookhaven National Laboratory, Upton, NY 11973.ORCID 0000-0002-1583-1291
Sabeeha S MerchantUCLA DOE Institute for Genomics and Proteomics, University of California, Los Angeles, CA 90095; sabeeha@berkeley.edu gallaher@chem.ucla.edu.ORCID 0000-0002-2594-509X

Funding

Howard Hughes Medical Institute
6 · The paper itself

Abstract

Polycistronic gene expression, common in prokaryotes, was thought to be extremely rare in eukaryotes. The development of long-read sequencing of full-length transcript isomers (Iso-Seq) has facilitated a reexamination of that dogma. Using Iso-Seq, we discovered hundreds of examples of polycistronic expression of nuclear genes in two divergent species of green algae:

Indexed as

Gene Expression Regulation, BacterialGene Expression Regulation, PlantChlorophytaOpen Reading FramesPlant ProteinsRNA, MessengerTranscription, GeneticPlant ProteinsRNA, Messengerbicistronicdicistronicleaky ribosome scanningtranscriptomeuORFs

Identifiers

PMID33579822
PMCPMC7896298

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.