ArticleGenome research2021
A minimally destructive protocol for DNA extraction from ancient teeth.
Article in Genome research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Teeth as a Post-Mortem DNA Source for Forensic Parentage Verification in Dogs: A Case Report.Veterinary sciences · 2026Article
- Local ancestry inference identifies robust evidence of selection in Neolithic Europe.Molecular biology and evolution · 2026Article
- Article
- Minimally Destructive Radiocarbon Dating of Bone.Rapid communications in mass spectrometry : RCM · 2026Article
- Ancient DNA from the Upper Paleolithic mammoth ivory of Hohle Fels, Germany.Scientific reports · 2026Article
- The persistence and loss of hard selective sweeps amid admixture in ancient Eurasians.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- On the Edge of Empire: Paleogenomic Insights into Roman Dacia.bioRxiv : the preprint server for biology · 2026Article
- The Genomic Legacy of the Norman Conquest in Rural England.bioRxiv : the preprint server for biology · 2026Article
- Genetic affinities between the ancient Greek colony of Amvrakia and its metropolis.Genome biology · 2026Article
- Cosmopolitanism in the depths of Barbaricum evidenced by archaeogenomic data from the Late Iron Age Goth community of the Masłomęcz group.Genome biology · 2026Article
- Review
- Global patterns of natural selection inferred using ancient DNA.bioRxiv : the preprint server for biology · 2026Article
- Article
- Continuity and discontinuity in Native American populations: Insights from ancient and modern mitochondrial DNA.Genetics and molecular biology · 2026Article
- Lessons learned: Recommendations for reproducible paleogenomic data analyses.American journal of human genetics · 2025Review
- Destructive and Non-Destructive Methods for aDNA Isolation from Teeth and Their Analysis: A Comparison.Genes · 2025Article
- The genetic history of Portugal over the past 5,000 years.Genome biology · 2025Article
- Review
- Ancient DNA reveals diverse community organizations in the 5th millennium BCE Carpathian Basin.Nature communications · 2025Article
- Urbanization and genetic homogenization in the medieval Low Countries revealed through a ten-century paleogenomic study of the city of Sint-Truiden.Genome biology · 2025Article
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Authors and funding
41 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Ancient DNA sampling methods-although optimized for efficient DNA extraction-are destructive, relying on drilling or cutting and powdering (parts of) bones and teeth. As the field of ancient DNA has grown, so have concerns about the impact of destructive sampling of the skeletal remains from which ancient DNA is obtained. Due to a particularly high concentration of endogenous DNA, the cementum of tooth roots is often targeted for ancient DNA sampling, but destructive sampling methods of the cementum often result in the loss of at least one entire root. Here, we present a minimally destructive method for extracting ancient DNA from dental cementum present on the surface of tooth roots. This method does not require destructive drilling or grinding, and, following extraction, the tooth remains safe to handle and suitable for most morphological studies, as well as other biochemical studies, such as radiocarbon dating. We extracted and sequenced ancient DNA from 30 teeth (and nine corresponding petrous bones) using this minimally destructive extraction method in addition to a typical tooth sampling method. We find that the minimally destructive method can provide ancient DNA that is of comparable quality to extracts produced from teeth that have undergone destructive sampling processes. Further, we find that a rigorous cleaning of the tooth surface combining diluted bleach and UV light irradiation seems sufficient to minimize external contaminants usually removed through the physical removal of a superficial layer when sampling through regular powdering methods.
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