ArticleMicrobial cell factories2021
Inducing secondary metabolite production of Aspergillus sydowii through microbial co-culture with Bacillus subtilis.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- Dual-functionMicrobiology spectrum · 2026Article
- Mass spectrometry-based metabolomics approaches to interrogate host-microbiome interactions in mammalian systems.Natural product reports · 2026Review
- From Molecules to Metabolomes, Understanding Symbiosis through Small Molecules.Journal of natural products · 2026Review
- Article
- Article
- Marine Fungal Metabolites: A Promising Source for Antibiofilm Compounds.Molecules (Basel, Switzerland) · 2025Review
- The impact of tropodithietic acid on microbial physiology under varying culture complexities.mSphere · 2025Article
- Metabolomic profiling and antimicrobial investigation ofNarra J · 2025Article
- Machine learning-optimized bioprocess for macroidin production by Lysinibacillus macroides and its biomedical applications.Bioprocess and biosystems engineering · 2025Article
- Bio-induced overproduction of heterocycloanthracin-like bacteriocin in Lysinibacillus macroides by Aspergillus austroafricanus: optimization of medium conditions and evaluation of potential applications.BMC biotechnology · 2025Article
- Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity.BMC genomics · 2025Article
- Newly isolated terpenoids (covering 2019-2024) from Aspergillus species and their potential for the discovery of novel antimicrobials.Natural products and bioprospecting · 2025Review
- The taxonomy ofFrontiers in microbiology · 2025Article
- A systematic approach to determine the outcome of the competition between two microbial species in bioreactor cocultures.Antonie van Leeuwenhoek · 2024Article
- Antimicrobial Activity ofAntibiotics (Basel, Switzerland) · 2024Article
- Chemical Epigenetic Regulation Secondary Metabolites Derived fromMolecules (Basel, Switzerland) · 2024Article
- Article
- Article
- Genomic and metabolomic insights into the antimicrobial compounds and plant growth-promoting potential of Bacillus velezensis Q-426.BMC genomics · 2023Article
- Mining Xanthine Oxidase Inhibitors from an Edible SeaweedMarine drugs · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe co-culture strategy which mimics natural ecology by constructing an artificial microbial community is a useful tool to activate the biosynthetic gene clusters to generate new metabolites. However, the conventional method to study the co-culture is to isolate and purify compounds separated by HPLC, which is inefficient and time-consuming. Furthermore, the overall changes in the metabolite profile cannot be well characterized.
resultsA new approach which integrates computational programs, MS-DIAL, MS-FINDER and web-based tools including GNPS and MetaboAnalyst, was developed to analyze and identify the metabolites of the co-culture of Aspergillus sydowii and Bacillus subtilis. A total of 25 newly biosynthesized metabolites were detected only in co-culture. The structures of the newly synthesized metabolites were elucidated, four of which were identified as novel compounds by the new approach. The accuracy of the new approach was confirmed by purification and NMR data analysis of 7 newly biosynthesized metabolites. The bioassay of newly synthesized metabolites showed that four of the compounds exhibited different degrees of PTP1b inhibitory activity, and compound N2 had the strongest inhibition activity with an IC
conclusionsCo-culture led to global changes of the metabolite profile and is an effective way to induce the biosynthesis of novel natural products. The new approach in this study is one of the effective and relatively accurate methods to characterize the changes of metabolite profiles and to identify novel compounds in co-culture systems.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.