Evidence map›Paper›PMID 33576966›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Visualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture.

Roshini Prakash, Nikita Shivani Thareja, Thomas S Carmichael, Raymond L Barnhill, Claire Lugassy, Laurent A Bentolila

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Roshini PrakashDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Nikita Shivani TharejaDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Thomas S CarmichaelDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Raymond L BarnhillInstitut Curie, Paris, France.
Claire LugassyDepartment of Translational Research, Institut Curie, Paris, France.
Laurent A BentolilaCalifornia NanoSystems Institute, University of California, Los Angeles, CA, USA. lbento@chem.ucla.edu.
University of California, Los Angeles · USInstitut Curie · FR

Funding

UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
NCATS NIH HHS UL1 TR000124NCATS NIH HHS UL1 TR001881
6 · The paper itself

Abstract

In addition to intravascular dissemination, angiotropic melanoma cells have the propensity to spread along the external surface of blood vessels in a pericytic location, or pericytic mimicry. Such continuous migration without intravasation has been termed "extravascular migratory metastasis" or EVMM. In order to visualize this mechanism of tumor propagation, we used a murine brain melanoma model utilizing green fluorescent human melanoma cells and red fluorescent lectin-tagged murine vessels. This model allows the direct microscopic visualization and mapping of the interaction of melanoma cells with the brain vasculature. In this chapter, we describe the methodology of lectin perfusion to label the entire angioarchitecture in conjunction with confocal microscopy imaging to study the pericyte mimicry of the angiotropic GFP+ melanoma cells.

Indexed as

AnimalsCell Line, TumorCell MovementFemaleGreen Fluorescent ProteinsImmunohistochemistryLectinsMaleMelanomaMiceMice, NudeMicroscopy, ConfocalNeoplasm InvasivenessNeovascularization, PathologicOptical ImagingPerfusionGreen Fluorescent ProteinsLectinsAngioarchitectureAngiotropismBlood vesselCancer cell migrationDermisEVMMLectinsMelanomaMetastasisPericyte mimicry

Identifiers

PMID33576966
PMCPMC8405202
OpenAlexW3126949528

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.