ArticleBrain pathology (Zurich, Switzerland)2021
Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.
Article in Brain pathology (Zurich, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Construction and Applicability Scenarios of 3D Neurovascular Unit Models In Vitro.Biomolecules · 2026Review
- Catalpol: an natural multifunctional iridoid glycoside with promising therapeutic properties.Frontiers in molecular biosciences · 2026Review
- Cognitive impairment after cerebral ischemia-reperfusion injury: a neuroecosystem perspective.Frontiers in neurology · 2026Review
- 3DFrontiers in pharmacology · 2025Review
- Cellular senescence in Alzheimer's disease: from physiology to pathology.Translational neurodegeneration · 2024Review
- Brain repair mechanisms after cell therapy for stroke.Brain : a journal of neurology · 2024Review
- The Progress in Molecular Transport and Therapeutic Development in Human Blood-Brain Barrier Models in Neurological Disorders.Cellular and molecular neurobiology · 2024Review
- Involvement of Astrocytes in the Formation, Maintenance, and Function of the Blood-Brain Barrier.Cells · 2024Review
- Combined administration of catalpol, puerarin, gastrodin, and borneol modulates the Tlr4/Myd88/NF-κB signaling pathway and alleviates microglia inflammation in Alzheimer's disease.Frontiers in pharmacology · 2024Article
- Endogenous Extracellular Vesicles Participate in Brain Remodeling after Ischemic Stroke.International journal of molecular sciences · 2023Article
- Catalpol Alleviates Ischemic Stroke Through Promoting Angiogenesis and Facilitating Proliferation and Differentiation of Neural Stem Cells via the VEGF-A/KDR Pathway.Molecular neurobiology · 2023Article
- Bioengineered perfused human brain microvascular networks enhance neural progenitor cell survival, neurogenesis, and maturation.Science advances · 2023Article
- Neural Stem Cells Therapy for Ischemic Stroke: Progress and Challenges.Translational stroke research · 2022Review
- Catalpol improves impaired neurovascular unit in ischemic stroke rats via enhancing VEGF-PI3K/AKT and VEGF-MEK1/2/ERK1/2 signaling.Acta pharmacologica Sinica · 2022Article
- Viable human brain microvessels for the study of aging and neurodegenerative diseases.Microvascular research · 2022Article
- Exercise improves choroid plexus epithelial cells metabolism to prevent glial cell-associated neurodegeneration.Frontiers in pharmacology · 2022Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Neurovascular dysfunction is a primary or secondary cause in the pathogenesis of several cerebrovascular and neurodegenerative disorders, including stroke. Therefore, the overall protection of the neurovascular unit (NVU) is a promising therapeutic strategy for various neurovascular diseases. However, the complexity of the NVU limits the study of the pathological mechanisms of neurovascular dysfunction. Reconstituting the in vitro NVU is important for the pathological study and drug screening of neurovascular diseases. In this study, we generated a spontaneously assembled three-dimensional NVU (3D NVU) by employing the primary neural stem cells and brain microvascular endothelial cells in a Matrigel extracellular matrix platform. This novel model exhibits the fundamental structures and features of the NVU, including neurons, astrocytes, oligodendrocytes, vascular-like structures, and blood-brain barrier-like characteristics. Additionally, under oxygen-glucose deprivation, the 3D NVU exhibits the neurovascular- or oxidative stress-related pathological characteristics of cerebral ischemia and the injuries can be mitigated, respectively, by supplementing with the vascular endothelial growth factor or edaravone, which demonstrated that the availability of 3D NVU in ischemic stroke modeling. Finally, the 3D NVU promoted the angiogenesis and neurogenesis in the brain of cerebral ischemia rats. We expect that the proposed in vitro 3D NVU model will be widely used to investigate the relationships between angiogenesis and neurogenesis and to study the pathology and pharmacology of neurovascular diseases.
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