GPU empowered pipelines for calculating genome-wide kinship matrices with ultra-high dimensional genetic variants and facilitating 1D and 2D GWAS.

Genome-wide association study (GWAS) is a powerful approach that has revolutionized the field of quantitative genetics. Two-dimensional GWAS that accounts for epistatic genetic effects needs to consider the effects of marker pairs, thus quadratic genetic variants, compared to one-dimensional GWAS that accounts for individual genetic variants. Calculating genome-wide kinship matrices in GWAS that account for relationships among individuals represented by ultra-high dimensional genetic variants is computationally challenging. Fortunately, kinship matrix calculation involves pure matrix operations and the algorithms can be parallelized, particular on graphics processing unit (GPU)-empowered high-performance computing (HPC) architectures. We have devised a new method and two pipelines: KMC1D and KMC2D for kinship matrix calculation with high-dimensional genetic variants, respectively, facilitating 1D and 2D GWAS analyses. We first divide the ultra-high-dimensional markers and marker pairs into successive blocks. We then calculate the kinship matrix for each block and merge together the block-wise kinship matrices to form the genome-wide kinship matrix. All the matrix operations have been parallelized using GPU kernels on our NVIDIA GPU-accelerated server platform. The performance analyses show that the calculation speed of KMC1D and KMC2D can be accelerated by 100-400 times over the conventional CPU-based computing.