ArticleFrontiers in veterinary science2021
A Multi-Platform Metabolomics Approach Identifies Urinary Metabolite Signatures That Differentiate Ketotic From Healthy Dairy Cows.
Article in Frontiers in veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Comparative metabolomic profiling of bovine urine using different sample processing methods by non-targeted GC-MS analysis.Veterinary research communications · 2026Article
- Applications of metabolomics in cow health assessment.Metabolomics : Official journal of the Metabolomic Society · 2025Review
- Insights into the Regulatory Effect of Danggui Buxue Tang in Postpartum Dairy Cows Through an Integrated Analysis of Multi-Omics and Network Analysis.Life (Basel, Switzerland) · 2025Article
- Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis-A Comparative Study.Animals : an open access journal from MDPI · 2025Article
- Identifying Predictive Biomarkers of Subclinical Mastitis in Dairy Cows through Urinary Metabotyping.Metabolites · 2024Article
- Screening for potential warning biomarkers in cows with ketosis based on host-microbiota co-metabolism analysis.Frontiers in microbiology · 2024Article
- Animal Wellness: The Power of Multiomics and Integrative Strategies: Multiomics in Improving Animal Health.Veterinary medicine international · 2024Review
- Predicting lameness in dairy cattle using untargeted liquid chromatography-mass spectrometry-based metabolomics and machine learning.Journal of dairy science · 2023Article
- A rapid LC-MS/MS assay for detection and monitoring of underivatized branched-chain amino acids in maple syrup urine disease.Journal of mass spectrometry and advances in the clinical lab · 2022Article
- Metabolic profiling of serum and urine in lactating dairy cows affected by subclinical ketosis using proton nuclear magnetic resonance spectroscopy.Journal of animal science and technology · 2022Article
- Metabolomics Analysis Across Multiple Biofluids Reveals the Metabolic Responses of Lactating Holstein Dairy Cows to Fermented Soybean Meal Replacement.Frontiers in veterinary science · 2022Article
- Preliminary demonstration of benchtop NMR metabolic profiling of feline urine: chronic kidney disease as a case study.BMC research notes · 2021Article
- Analytical Methodology for a Metabolome Atlas of Goat's Plasma, Milk and Feces UsingMetabolites · 2021Article
- A Targeted Serum Metabolomics GC-MS Approach Identifies Predictive Blood Biomarkers for Retained Placenta in Holstein Dairy Cows.Metabolites · 2021Article
- Differentiation of Subclinical Ketosis and Liver Function Test Indices in Adipose Tissues Associated With Hyperketonemia in Postpartum Dairy Cattle.Frontiers in veterinary science · 2021Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ketosis and subclinical ketosis are widespread among dairy cows especially after calving. Etiopathology of ketosis has been related to negative energy balance. The objective of this study was to investigate metabolite fingerprints in the urine of pre-ketotic, ketotic, and post-ketotic cows to identify potential metabolite alterations that can be used in the future to identify susceptible cows for ketosis and metabolic pathways involved in the development of disease. In this study, NMR, DI/LC-MS/MS, and GC-MS-based metabolomics were used to analyze urine samples from 6 cows diagnosed with ketosis and 20 healthy control (CON) cows at -8 and -4 weeks prepartum, the week (+1 to +3) of ketosis diagnosis, and at +4 and +8 weeks after parturition. Univariate and multivariate analyses were used to screen metabolite panels that can identify cows at their pre-ketotic stage. A total of 54, 42, 48, 16, and 31 differential metabolites between the ketotic and CON cows were identified at -8 and -4 weeks prepartum, ketosis week, and at +4, and +8 weeks postpartum, respectively. Variable importance in projection (VIP) plots ranked the most significant differential metabolites, which differentiated ketotic cows from the CON ones. Additionally, several metabolic pathways that are related to ketosis were identified. Moreover, two promising metabolite panels were identified which clearly separated ketotic from CON cows with excellent level of sensitivity and specificity. Overall, multiple urinary metabolite alterations were identified in pre-ketotic, ketotic, and post-ketotic cows. The metabolite panels identified need to be validated in the future in a larger cohort of animals.
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