Evidence map›Paper›PMID 33574459›Full record

ArticleScientific reports2021

Optical vagus nerve modulation of heart and respiration via heart-injected retrograde AAV.

Arjun K Fontaine, Gregory L Futia, Pradeep S Rajendran, Samuel F Littich, Naoko Mizoguchi, Kalyanam Shivkumar, Jeffrey L Ardell, Diego Restrepo, John H Caldwell, Emily A Gibson and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Arjun K Fontaine *Departments of Bioengineering, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA. arjun.fontaine@cuanschutz.edu.
Gregory L Futia *Departments of Bioengineering, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
Pradeep S RajendranUCLA Cardiac Arrhythmia Center, University of California Los Angeles, Los Angeles, CA, USA.
Samuel F LittichDepartments of Bioengineering, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
Naoko MizoguchiDepartments of Cell and Developmental Biology, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
Kalyanam ShivkumarUCLA Cardiac Arrhythmia Center, University of California Los Angeles, Los Angeles, CA, USA.
Jeffrey L ArdellUCLA Cardiac Arrhythmia Center, University of California Los Angeles, Los Angeles, CA, USA.
Diego RestrepoDepartments of Cell and Developmental Biology, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
John H CaldwellDepartments of Cell and Developmental Biology, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
Emily A GibsonDepartments of Bioengineering, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
Richard F Ff WeirDepartments of Bioengineering, University of Colorado - Anschutz Medical Campus, Aurora, CO, USA.
University of Colorado Anschutz Medical Campus · USUniversity of California, Los Angeles · USMeikai University · JP

Funding

University of Colorado Anschutz Medical Campus DRCP30DK116073 · NIDDK · UNIVERSITY OF COLORADO DENVER · PI LORI SUSSEL · 2020 to 2026
$10.8M
ZEBRAFISH TRANSGENIC CORE - ROCKY MOUNTAIN NEUROLOGICAL DISORDERS CORE CENTERP30NS048154 · NINDS · UNIVERSITY OF COLORADO DENVER · PI RIBERA, ANGELES BADELL · 2004 to 2020
$10.1M
Optimization of a Minimally-Invasive Bidirectional Optogenetic Peripheral Nerve Interface with Single Axon Read-in & Read-out SpecificityR01NS118188 · NINDS · UNIVERSITY OF COLORADO DENVER · PI CALDWELL, JOHN H, GIBSON, EMILY · 2020 to 2024
$3.3M
Controlled neuronal firing in vivo using two photon spatially shaped optogeneticsU01NS099577 · NINDS · UNIVERSITY OF COLORADO DENVER · PI GIBSON, EMILY, RESTREPO, DIEGO · 2017 to 2019
$2.3M
Development of a Bidirectional Optogenetic Minimally Invasive Peripheral Nerve Interface with Single Axon Read-in & Read-out SpecificityOT2OD023852 · OD · UNIVERSITY OF COLORADO DENVER · PI CALDWELL, JOHN H, GIBSON, EMILY · 2016 to 2018
$2.1M
Neuronal Transdifferention in vivo: Mechanism and PotentialR01NS086839 · NINDS · UNIVERSITY OF COLORADO DENVER · PI RIBERA, ANGELES BADELL · 2014 to 2018
$1.7M
NIDDK NIH HHS P30 DK116073NIH HHS OT2 OD023852NINDS NIH HHS P30 NS048154NINDS NIH HHS R01 NS086839NINDS NIH HHS R01 NS118188NINDS NIH HHS U01 NS099577RRD VA IK6 RX002996
6 · The paper itself

Abstract

Vagus nerve stimulation has shown many benefits for disease therapies but current approaches involve imprecise electrical stimulation that gives rise to off-target effects, while the functionally relevant pathways remain poorly understood. One method to overcome these limitations is the use of optogenetic techniques, which facilitate targeted neural communication with light-sensitive actuators (opsins) and can be targeted to organs of interest based on the location of viral delivery. Here, we tested whether retrograde adeno-associated virus (rAAV2-retro) injected in the heart can be used to selectively express opsins in vagus nerve fibers controlling cardiac function. Furthermore, we investigated whether perturbations in cardiac function could be achieved with photostimulation at the cervical vagus nerve. Viral injection in the heart resulted in robust, primarily afferent, opsin reporter expression in the vagus nerve, nodose ganglion, and brainstem. Photostimulation using both one-photon stimulation and two-photon holography with a GRIN-lens incorporated nerve cuff, was tested on the pilot-cohort of injected mice. Changes in heart rate, surface electrocardiogram, and respiratory responses were observed in response to both one- and two-photon photostimulation. The results demonstrate feasibility of retrograde labeling for organ targeted optical neuromodulation.

Indexed as

AnimalsDependovirusElectric StimulationHeartHeart RateHumansMiceNeuronsOpsinsOptogeneticsRespirationVagus NerveVagus Nerve StimulationOpsins

Identifiers

PMID33574459
PMCPMC7878800
OpenAlexW3126344284

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.