Evidence map›Paper›PMID 33574458›Full record

ArticleScientific reports2021

Direct reprogramming of oligodendrocyte precursor cells into GABAergic inhibitory neurons by a single homeodomain transcription factor Dlx2.

Linda L Boshans, Heun Soh, William M Wood, Timothy M Nolan, Ion I Mandoiu, Yuchio Yanagawa, Anastasios V Tzingounis, Akiko Nishiyama

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. The Many Lives of an Oligodendrocyte Precursor Cell.Annual review of neuroscience · 2025
    Review
  6. Article
  7. Review
  8. Review
  9. Article
  10. Review
  11. Article
  12. The epigenetic landscape of oligodendrocyte lineage cells.Annals of the New York Academy of Sciences · 2023
    Review
  13. Review
  14. NG2-glia crosstalk with microglia in health and disease.CNS neuroscience & therapeutics · 2022
    Review
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Linda L BoshansDepartment of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA.
Heun SohDepartment of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA.
William M WoodDepartment of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA.
Timothy M NolanDepartment of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA.
Ion I MandoiuDepartment of Computer Science and Engineering, University of Connecticut, Storrs, CT, USA.
Yuchio YanagawaDepartment of Genetic and Behavioral Neuroscience, Gunma University Graduate School of Medicine, Maebashi, Japan.
Anastasios V TzingounisDepartment of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA.
Akiko NishiyamaDepartment of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA. akiko.nishiyama@uconn.edu.
University of Connecticut · USGunma University · JP

Funding

KCNQ2/3 channels and interneuron physiologyR01NS101596 · NINDS · UNIVERSITY OF CONNECTICUT STORRS · PI TZINGOUNIS, ANASTASIOS · 2017 to 2021
$1.7M
Heterogeneity of NG2 Glial CellsR01NS074870 · NINDS · UNIVERSITY OF CONNECTICUT STORRS · PI NISHIYAMA, AKIKO · 2012 to 2016
$1.7M
Leica TCS SP8 FSU AOBS 405 UV Spectral Confocal MicroscopeS10OD016435 · OD · UNIVERSITY OF CONNECTICUT STORRS · PI NISHIYAMA, AKIKO · 2014 to 2014
$456k
NIH HHS S10 OD016435NINDS NIH HHS R01 NS074870NINDS NIH HHS R01 NS101596
6 · The paper itself

Abstract

Oligodendrocyte precursor cells (NG2 glia) are uniformly distributed proliferative cells in the mammalian central nervous system and generate myelinating oligodendrocytes throughout life. A subpopulation of OPCs in the neocortex arises from progenitor cells in the embryonic ganglionic eminences that also produce inhibitory neurons. The neuronal fate of some progenitor cells is sealed before birth as they become committed to the oligodendrocyte lineage, marked by sustained expression of the oligodendrocyte transcription factor Olig2, which represses the interneuron transcription factor Dlx2. Here we show that misexpression of Dlx2 alone in postnatal mouse OPCs caused them to switch their fate to GABAergic neurons within 2 days by downregulating Olig2 and upregulating a network of inhibitory neuron transcripts. After two weeks, some OPC-derived neurons generated trains of action potentials and formed clusters of GABAergic synaptic proteins. Our study revealed that the developmental molecular logic can be applied to promote neuronal reprogramming from OPCs.

Indexed as

Cell ProliferationCellular ReprogrammingCentral Nervous SystemEmbryonic DevelopmentGABAergic NeuronsGene Expression RegulationHomeodomain ProteinsNeurogliaOligodendrocyte Precursor CellsOligodendrocyte Transcription Factor 2SynapsesTranscription FactorsDistal-less homeobox proteinsHomeodomain ProteinsOlig2 protein, mouseOligodendrocyte Transcription Factor 2Transcription Factors

Identifiers

PMID33574458
PMCPMC7878775
OpenAlexW3128101862

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.