ArticleNature communications2021
Mechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Inosine monophosphate dehydrogenases are key players for functional development in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Crystallographic snapshots of Trypanosoma cruzi aspartate transcarbamoylase including catalytic intermediates suggest an ordered Bi-Bi reaction mechanism.Scientific reports · 2026Article
- Repurposing of Inhibitors of Plasmodial Aspartate Transcarbamoylase Toward Trypanosoma Cruzi.ChemMedChem · 2026Article
- Topological criterion for robust perfect adaptation of reaction fluxes in biological networks.iScience · 2025Article
- Targeted metabolic engineering of key biosynthetic genes improves triptolide production in Tripterygium wilfordii hairy roots.Plant cell reports · 2025Article
- Metabonomics and physiology revealed the critical function of 5-Phosphoribosylamine and antioxidant enzymes in enhancing aged oat seed germination.BMC plant biology · 2025Article
- Human milk-derived 5'-UMP promotes thermogenesis and mitochondrial biogenesis to ameliorate obesity.Frontiers in nutrition · 2025Article
- Three Arabidopsis UMP kinases have different roles in pyrimidine nucleotide biosynthesis and (deoxy)CMP salvage.The Plant cell · 2024Article
- Nucleotide Imbalance, Provoked by Downregulation of Aspartate Transcarbamoylase Impairs Cold Acclimation in Arabidopsis.Molecules (Basel, Switzerland) · 2023Article
- A Tailored Strategy to Crosslink the Aspartate Transcarbamoylase Domain of the Multienzymatic Protein CAD.Molecules (Basel, Switzerland) · 2023Article
- A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis.The Plant cell · 2023Article
- Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings.Frontiers in plant science · 2023Article
- Structural analysis and molecular substrate recognition properties ofFrontiers in plant science · 2023Article
- New Insight into Aspartate Metabolic Pathways inInternational journal of molecular sciences · 2022Article
- Cytidine Triphosphate Synthase Four FromFrontiers in plant science · 2022Article
- Genome and transcriptome-wide study of carbamoyltransferase genes in major fleshy fruits: A multi-omics study of evolution and functional significance.Frontiers in plant science · 2022Article
- Deciphering CAD: Structure and function of a mega-enzymatic pyrimidine factory in health and disease.Protein science : a publication of the Protein Society · 2021Review
- Cytosolic CTP Production Limits the Establishment of Photosynthesis inFrontiers in plant science · 2021Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aspartate transcarbamoylase (ATC), an essential enzyme for de novo pyrimidine biosynthesis, is uniquely regulated in plants by feedback inhibition of uridine 5-monophosphate (UMP). Despite its importance in plant growth, the structure of this UMP-controlled ATC and the regulatory mechanism remain unknown. Here, we report the crystal structures of Arabidopsis ATC trimer free and bound to UMP, complexed to a transition-state analog or bearing a mutation that turns the enzyme insensitive to UMP. We found that UMP binds and blocks the ATC active site, directly competing with the binding of the substrates. We also prove that UMP recognition relies on a loop exclusively conserved in plants that is also responsible for the sequential firing of the active sites. In this work, we describe unique regulatory and catalytic properties of plant ATCs that could be exploited to modulate de novo pyrimidine synthesis and plant growth.
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