ArticlePlant physiology2021
In vivo single-particle tracking of the aquaporin AtPIP2;1 in stomata reveals cell type-specific dynamics.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- FIMBRIN2 Regulates ABA-Induced Stomatal Closure by Promoting Microfilament Bundling and Turnover in Arabidopsis Guard Cells.Plant direct · 2026Article
- Post-translational modification acts as a digital like switch influencing AtPIP2;1 water and cation permeability.Scientific reports · 2025Article
- Real-Time Observation of Polymer Fluctuations During Phase Transition Using Transmission Electron Microscope.Polymers · 2025Article
- Promoter of Vegetable PeaInternational journal of molecular sciences · 2024Article
- Putting Biochar in Action: A Black Gold for Efficient Mitigation of Salinity Stress in Plants. Review and Future Directions.ACS omega · 2024Review
- Protein Disorder in Plant Stress Adaptation: From Late Embryogenesis Abundant to Other Intrinsically Disordered Proteins.International journal of molecular sciences · 2024Review
- Microtubule Regulation in Plants: From Morphological Development to Stress Adaptation.Biomolecules · 2023Review
- Membrane Dynamics Regulated by Cytoskeleton in Plant Immunity.International journal of molecular sciences · 2023Review
- The critical role of biochar to mitigate the adverse impacts of drought and salinity stress in plants.Frontiers in plant science · 2023Review
- Exploring aquaporin functions during changes in leaf water potential.Frontiers in plant science · 2023Review
- Transcriptomic and co-expression network analyses on diverse wheat landraces identifies candidate master regulators of the response to early drought.Frontiers in plant science · 2023Article
- A review for cell and particle tracking on microscopy images using algorithms and deep learning technologies.Biomedical journal · 2022Article
- Transcription Profiles Reveal Age-Dependent Variations of Photosynthetic Properties and Sugar Metabolism in Grape Leaves (International journal of molecular sciences · 2022Article
- Local endocytosis of sucrose transporter 2 in duckweed reveals the role of sucrose transporter 2 in guard cells.Frontiers in plant science · 2022Article
- Environmental Cues Contribute to Dynamic Plasma Membrane Organization of Nanodomains Containing Flotillin-1 and Hypersensitive Induced Reaction-1 Proteins inFrontiers in plant science · 2022Article
- Controlling the Gate: The Functions of the Cytoskeleton in Stomatal Movement.Frontiers in plant science · 2022Review
- Bioimaging tools move plant physiology studies forward.Frontiers in plant science · 2022Article
- Hormonal and environmental signaling pathways target membrane water transport.Plant physiology · 2021Review
- Membrane nanodomains and transport functions in plant.Plant physiology · 2021Review
- Deciphering the role of microRNAs duringPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aquaporins such as the plasma membrane intrinsic proteins (PIPs) allow water to move through cell membranes and are vital for stomatal movement in plants. Despite their importance, the dynamic changes in aquaporins during water efflux and influx have not been directly observed in real time in vivo. Here, to determine which factors regulate these changes during the bidirectional translocation of water, we examined aquaporin dynamics during the stomatal immune response to the bacterial flagellin-derived peptide flg22. The Arabidopsis (Arabidopsis thaliana) aquaporin mutant pip2;1 showed defects in the flg22-induced stomatal response. Variable-angle total internal reflection fluorescence microscopy revealed that the movement dynamics and dwell times of AQ6]GFP-AtPIP2;1 in guard cells and subsidiary cells exhibited cell type-specific dependencies on flg22. The cytoskeleton, rather than the cell wall, was the major factor regulating AtPIP2;1 dynamics, although both the cytoskeleton and cell wall might form bounded domains that restrict the diffusion of AtPIP2;1 in guard cells and subsidiary cells. Finally, our analysis revealed the different roles of cortical actin and microtubules in regulating AtPIP2;1 dynamics in guard cells, as well as subsidiary cells, under various conditions. Our observations shed light on the heterogeneous mechanisms that regulate membrane protein dynamics in plants in response to pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.