ArticleScientific reports2021
Submicron spatial resolution optical coherence tomography for visualising the 3D structures of cells cultivated in complex culture systems.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- The Machine Learning Classification of Retinal Ganglion Cell Dendritic Texture in a 3xTg-Alzheimer's Disease Mouse Model.Diagnostics (Basel, Switzerland) · 2026Article
- Anterior Segment Optical Coherence Tomography Epithelial Thickness Mapping for Lesion Delineation in Recurrent Corneal Erosion: A Paired-Method Study.Translational vision science & technology · 2026Article
- Anterior Segment OCT Thickness Difference Maps for Monitoring Corneal Thickness Changes: Small-Incision Lenticule Extraction as a Model.Translational vision science & technology · 2025Article
- SVM-Based Optical Detection of Retinal Ganglion Cell Apoptosis.Photonics · 2025Article
- Nanosensitive optical coherence tomography for detecting structural changes in stem cells.Biomedical optics express · 2023Article
- Optical Coherence Tomography Is a Promising Tool for Zebrafish-Based Research-A Review.Bioengineering (Basel, Switzerland) · 2022Review
- Methods and applications of full-field optical coherence tomography: a review.Journal of biomedical optics · 2022Review
- Robotic-arm-assisted flexible large field-of-view optical coherence tomography.Biomedical optics express · 2021Article
- Development of a high-resolution three-dimensional optical coherence tomography for characterization of epithelial cell products for corneal cell therapy.Taiwan journal of ophthalmologyArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three-dimensional (3D) configuration of in vitro cultivated cells has been recognised as a valuable tool in developing stem cell and cancer cell therapy. However, currently available imaging approaches for live cells have drawbacks, including unsatisfactory resolution, lack of cross-sectional and 3D images, and poor penetration of multi-layered cell products, especially when cells are cultivated on semitransparent carriers. Herein, we report a prototype of a full-field optical coherence tomography (FF-OCT) system with isotropic submicron spatial resolution in en face and cross-sectional views that provides a label-free, non-invasive platform with high-resolution 3D imaging. We validated the imaging power of this prototype by examining (1) cultivated neuron cells (N2A cell line); (2) multilayered, cultivated limbal epithelial sheets (mCLESs); (3) neuron cells (N2A cell line) and mCLESs cultivated on a semitransparent amniotic membrane (stAM); and (4) directly adherent colonies of neuron-like cells (DACNs) covered by limbal epithelial cell sheets. Our FF-OCT exhibited a penetrance of up to 150 μm in a multilayered cell sheet and displayed the morphological differences of neurons and epithelial cells in complex coculture systems. This FF-OCT is expected to facilitate the visualisation of cultivated cell products in vitro and has a high potential for cell therapy and translational medicine research.
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