ArticleScientific reports2021
Rat in vitro spermatogenesis promoted by chemical supplementations and oxygen-tension control.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Platelet-rich plasma (PRP) and the future of male fertility: a path forward for personalized and regenerative therapies.Stem cell research & therapy · 2025Pooled it
- Platelet-rich plasma hydrogel supports long-term germ cell maintenance in rat testicular organotypic culture.Journal of assisted reproduction and genetics · 2026Article
- Reverse transcriptase inhibitors enable the generation of fertile spermatids from fetal mouse testes in vitro.Communications biology · 2026Article
- A simplified two-dimensional culture system supports meiotic progression during mouse spermatogenesis in vitro.PloS one · 2026Article
- Differential retinoic acid responses across testicular developmentbioRxiv : the preprint server for biology · 2025Article
- In vitro models of the male reproductive system: applications for developmental and reproductive toxicology.Toxicological sciences : an official journal of the Society of Toxicology · 2025Review
- Microfluidic systems in testicular in vitro culture: a powerful model tool for spermatogenesis and reprotoxicity studies.Asian journal of andrology · 2025Review
- Artificial Gametogenesis and In Vitro Spermatogenesis: Emerging Strategies for the Treatment of Male Infertility.International journal of molecular sciences · 2025Review
- Article
- Meeting proceedings: International Society for Fertility Preservation Tokyo, 15-17 November, 2024.Journal of assisted reproduction and genetics · 2025Article
- 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization.Advanced healthcare materials · 2025Article
- In vitro sperm generation from immature mouse testicular tissue using plasma rich in growth factors.Stem cell research & therapy · 2025Article
- Development of the membrane ceiling method for in vitro spermatogenesis.Scientific reports · 2025Article
- Organotypic culture system for prepubertal mice testicular tissue: A comparative study.Frontiers in endocrinology · 2025Article
- Spermatogonial stem cell technologies: applications from human medicine to wildlife conservation†.Biology of reproduction · 2024Review
- Application of testicular organ culture system for the evaluation of spermatogenesis impairment.Scientific reports · 2024Article
- Recent Progress of Induced Spermatogenesis In Vitro.International journal of molecular sciences · 2024Review
- In Vitro Generation of Haploid Germ Cells from Human XY and XXY Immature Testes in a 3D Organoid System.Bioengineering (Basel, Switzerland) · 2024Article
- Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control.The Journal of reproduction and development · 2024Article
- Human in vitro spermatogenesis as a regenerative therapy - where do we stand?Nature reviews. Urology · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro spermatogenesis (IVS) using air-liquid interphase organ culture method is possible with mouse testis tissues. The same method, however, has been hardly applicable to animals other than mice, only producing no or limited progression of spermatogenesis. In the present study, we challenged IVS of rats with modifications of culture medium, by supplementing chemical substances, including hormones, antioxidants, and lysophospholipids. In addition, reducing oxygen tension by placing tissues in an incubator of lower oxygen concentration and/or applying silicone cover ceiling on top of the tissue were effective for improving the spermatogenic efficiency. Through these modifications of the culture condition, rat spermatogenesis up to round spermatids was maintained over 70 days in the cultured tissue. Present results demonstrated a significant progress in rat IVS, revealing conditions commonly favorable for mice and rats as well as finding rat-specific optimizations. This is an important step towards successful IVS in many animal species, including humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.