Evidence map›Paper›PMID 33568644›Full record

ArticleNature communications2021

Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria.

Zhe Sun, Alexander V Yakhnin, Peter C FitzGerald, Carl E Mclntosh, Mikhail Kashlev

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Review
  3. Promoter proximal pausing of RNA polymerase across evolution.Nature structural & molecular biology · 2026
    Article
  4. Article
  5. Article
  6. Sticking around: σ factor dynamics and implications for transcription elongation.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  7. Article
  8. The sigma factor σFrontiers in microbiology · 2025
    Article
  9. Article
  10. Article
  11. Article
  12. Robust regulation of transcription pausing inProceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  13. Article
  14. Article
  15. Article
  16. Structural and mechanistic basis of σ-dependent transcriptional pausing.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Zhe SunRNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.ORCID 0000-0002-0634-7983
Alexander V YakhninRNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.ORCID 0000-0002-7313-7054
Peter C FitzGeraldGenome Analysis Unit, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID 0000-0001-8769-0507
Carl E MclntoshGenome Analysis Unit, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Mikhail KashlevRNA Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA. kashlevm@mail.nih.gov.ORCID 0000-0002-1260-6486
National Institutes of Health · US

Funding

Mechanisms of transcription pausing and fidelity in prokaryotes and eukaryotesZIABC010795 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI KASHLEV, MIKHAIL · 2009 to 2025
$18.1M
6 · The paper itself

Abstract

Promoter-proximal pausing regulates eukaryotic gene expression and serves as checkpoints to assemble elongation/splicing machinery. Little is known how broadly this type of pausing regulates transcription in bacteria. We apply nascent elongating transcript sequencing combined with RNase I footprinting for genome-wide analysis of σ

Indexed as

Transcription, GeneticDNA-Directed RNA PolymerasesEscherichia coliEscherichia coli ProteinsGene Expression Regulation, BacterialPromoter Regions, GeneticRNA, BacterialSequence Analysis, RNASigma FactorTranscriptional Elongation FactorsTranscription FactorsDNA-Directed RNA PolymerasesEscherichia coli ProteinsGreA protein, E coliGreB protein, E coliRNA, BacterialRNA polymerase sigma 70Sigma FactorTranscriptional Elongation FactorsTranscription Factors

Identifiers

PMID33568644
PMCPMC7876045
OpenAlexW3128445716

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.