ArticleBMC biology2021
Hidden genomic features of an invasive malaria vector, Anopheles stephensi, revealed by a chromosome-level genome assembly.
Article in BMC biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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26 citing papers in PubMed, 123 citations in OpenAlex.
- Multiple genomic approaches reveal geographic structure and local selection signals in invasive Anopheles stephensi from the Horn of Africa and Yemen.BMC genomics · 2026Article
- Two neuropeptides that promote blood feeding ineLife · 2026Article
- Article
- Increased heterozygosity and balancing selection in isofemale generations of Anopheles stephensi under laboratory conditions.BMC genomics · 2025Article
- Genome Structural Variants Shape Adaptive Success of an Invasive Urban Malaria Vector Anopheles stephensi.Molecular biology and evolution · 2025Article
- Genome wide population genetics and molecular surveillance of insecticide resistance in Anopheles stephensi mosquitoes from Awash Sebat Kilo in Ethiopia.Scientific reports · 2025Article
- The origin, invasion history and resistance architecture ofbioRxiv : the preprint server for biology · 2025Article
- Protective antibodies target cryptic epitope unmasked by cleavage of malaria sporozoite protein.Science (New York, N.Y.) · 2025Article
- Establishing a dominant early larval sex-selection strain in the Asian malaria vector Anopheles stephensi.Infectious diseases of poverty · 2024Article
- A decade of invasive Anopheles stephensi sequence-based identification: toward a global standard.Trends in parasitology · 2024Review
- The chromosome-scale genome assembly for the West Nile vector Culex quinquefasciatus uncovers patterns of genome evolution in mosquitoes.BMC biology · 2024Article
- Current Status of Omics Studies Elucidating the Features of Reproductive Biology in Blood-Feeding Insects.Insects · 2023Review
- Tracking Adaptive Pathways of Invasive Insects: Novel Insight from Genomics.International journal of molecular sciences · 2023Review
- Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay.Scientific reports · 2023Article
- Mapping current and future thermal limits to suitability for malaria transmission by the invasive mosquito Anopheles stephensi.Malaria journal · 2023Article
- Novel genome sequences and evolutionary dynamics of the North American anopheline species Anopheles freeborni, Anopheles crucians, Anopheles quadrimaculatus, and Anopheles albimanus.G3 (Bethesda, Md.) · 2023Article
- Diversity, duplication, and genomic organization of homeobox genes in Lepidoptera.Genome research · 2023Article
- A chromosome-level assembly of the widely used Rockefeller strain of Aedes aegypti, the yellow fever mosquito.G3 (Bethesda, Md.) · 2022Article
- Tandem duplication of a genomic region encoding glutathione S-transferase epsilon-2 and -4 genes in DDT-resistant Anopheles stephensi strain from India.Scientific reports · 2022Article
- Selective targeting of biting females to control mosquito-borne infectious diseases.Trends in parasitology · 2022Review
Corrections and comments
- Erratum issued
Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe mosquito Anopheles stephensi is a vector of urban malaria in Asia that recently invaded Africa. Studying the genetic basis of vectorial capacity and engineering genetic interventions are both impeded by limitations of a vector's genome assembly. The existing assemblies of An. stephensi are draft-quality and contain thousands of sequence gaps, potentially missing genetic elements important for its biology and evolution.
resultsTo access previously intractable genomic regions, we generated a reference-grade genome assembly and full transcript annotations that achieve a new standard for reference genomes of disease vectors. Here, we report novel species-specific transposable element (TE) families and insertions in functional genetic elements, demonstrating the widespread role of TEs in genome evolution and phenotypic variation. We discovered 29 previously hidden members of insecticide resistance genes, uncovering new candidate genetic elements for the widespread insecticide resistance observed in An. stephensi. We identified 2.4 Mb of the Y chromosome and seven new male-linked gene candidates, representing the most extensive coverage of the Y chromosome in any mosquito. By tracking full-length mRNA for > 15 days following blood feeding, we discover distinct roles of previously uncharacterized genes in blood metabolism and female reproduction. The Y-linked heterochromatin landscape reveals extensive accumulation of long-terminal repeat retrotransposons throughout the evolution and degeneration of this chromosome. Finally, we identify a novel Y-linked putative transcription factor that is expressed constitutively throughout male development and adulthood, suggesting an important role.
conclusionCollectively, these results and resources underscore the significance of previously hidden genomic elements in the biology of malaria mosquitoes and will accelerate the development of genetic control strategies of malaria transmission.
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