ArticlePloS one2021
A set of shuttle plasmids for gene expression in Acinetobacter baumannii.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- A non-antibiotic genetic selection system enables near-infrared in vivo imaging and evaluation of antibiotic efficacy for multidrug-resistant Acinetobacter baumannii.The Journal of antibiotics · 2026Article
- Nanopore sequencing in suitcase lab enables improved detection ofFrontiers in microbiology · 2026Article
- Development of two compatible plasmids to assess sRNA-mediated post-transcriptional regulation inMicrobiology (Reading, England) · 2025Article
- Mobilizable shuttle vectors with fluorescent markers functional across different species of bacteria.Applied and environmental microbiology · 2025Article
- Modular, inducible, and titratable expression systems forMicrobiology spectrum · 2024Article
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- Modular, inducible, and titratable expression systems forbioRxiv : the preprint server for biology · 2024Article
- Fisetin inhibitsMicrobiology spectrum · 2024Article
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- AspFlex: Molecular Tools to Study Gene Expression and Regulation inACS synthetic biology · 2023Article
- Discovery of Bactericidal Proteins fromAntibiotics (Basel, Switzerland) · 2023Article
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- Pandrug-resistantFrontiers in cellular and infection microbiology · 2023Article
- Polyphosphate Kinase Is Required for the Processes of Virulence and Persistence in Acinetobacter baumannii.Microbiology spectrum · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infections caused by the emerging opportunistic bacterial pathogen Acinetobacter baumannii are occurring at increasingly alarming rates, and such increase in incidence is further compounded by the development of wide spread multidrug-resistant strains. Yet, our understanding of its pathogenesis and biology remains limited which can be attributed in part to the scarce of tools for molecular genetic analysis of this bacterium. Plasmids based on pWH1277 originally isolated from Acinetobacter calcoaceticus are the only vehicles currently available for ectopic gene expression in Acinetobacter species, which restricts experiments that require simultaneous analysis of multiple genes. Here, we found that plasmids of the IncQ group are able to replicate in A. baumannii and can stably co-reside with derivatives of pWH1277. Furthermore, we have constructed a series of four plasmids that allow inducible expression of Flag-tagged proteins in A. baumannii by arabinose or isopropyl β-d-1-thiogalactopyranoside. Together with constructs previously developed, these plasmids will accommodate the need in genetic analysis of this increasingly important pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.