Evidence map›Paper›PMID 33566727›Full record

ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2021

Human placental-derived stem cell therapy ameliorates experimental necrotizing enterocolitis.

Victoria G Weis, Anna C Deal, Gehad Mekkey, Cara Clouse, Michaela Gaffley, Emily Whitaker, Cole B Peeler, Jared A Weis, Marshall Z Schwartz, Anthony Atala

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Gastrointestinal and liver physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Victoria G WeisWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Anna C DealWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Gehad MekkeyWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Cara ClouseWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Michaela GaffleyWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Emily WhitakerWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Cole B PeelerWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Jared A WeisSchool of Biomedical Engineering and Sciences, Virginia Tech-Wake Forest University, Blacksburg, Virginia.
Marshall Z SchwartzWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Anthony AtalaWake Forest Institute for Regenerative Medicine, Winston-Salem, North Carolina.
Wake Forest University · USForest Institute · USVirginia Tech - Wake Forest University School of Biomedical Engineering & Sciences · US

Funding

Multi-Scale Biomechanical Modeling of Tumor Growth and Response in Breast CancerK25CA204599 · NCI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI WEIS, JARED ANTHONY · 2016 to 2020
$747k
HHS | NIH | National Cancer Institute (NCI) K25CA204599NCI NIH HHS K25 CA204599
6 · The paper itself

Abstract

Necrotizing enterocolitis (NEC), a life-threatening intestinal disease, is becoming a larger proportionate cause of morbidity and mortality in premature infants. To date, therapeutic options remain elusive. Based on recent cell therapy studies, we investigated the effect of a human placental-derived stem cell (hPSC) therapy on intestinal damage in an experimental NEC rat pup model. NEC was induced in newborn Sprague-Dawley rat pups for 4 days via formula feeding, hypoxia, and LPS. NEC pups received intraperitoneal (ip) injections of either saline or hPSC (NEC-hPSC) at 32 and 56 h into NEC induction. At 4 days, intestinal macroscopic and histological damage, epithelial cell composition, and inflammatory marker expression of the ileum were assessed. Breastfed (BF) littermates were used as controls. NEC pups developed significant bowel dilation and fragility in the ileum. Further, NEC induced loss of normal villi-crypt morphology, disruption of epithelial proliferation and apoptosis, and loss of critical progenitor/stem cell and Paneth cell populations in the crypt. hPSC treatment improved macroscopic intestinal health with reduced ileal dilation and fragility. Histologically, hPSC administration had a significant reparative effect on the villi-crypt morphology and epithelium. In addition to a trend of decreased inflammatory marker expression, hPSC-NEC pups had increased epithelial proliferation and decreased apoptosis when compared with NEC littermates. Further, the intestinal stem cell and crypt niche that include Paneth cells, SOX9

Indexed as

ApoptosisCell ProliferationStem Cell TransplantationAnimalsAnimals, NewbornCells, CulturedCytokinesDisease Models, AnimalEnterocolitis, NecrotizingFemaleHumansIleumInflammation MediatorsIntestinal MucosaPaneth CellsPlacentaCytokinesInflammation MediatorsLgr5 protein, ratReceptors, G-Protein-CoupledSox9 protein, ratSOX9 Transcription FactorileumLGR5+ stem cellnecrotizing enterocolitisneonatalstem cell therapy

Identifiers

PMID33566727
PMCPMC8238163
OpenAlexW3128421204

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.