Evidence map›Paper›PMID 33566163›Full record

ArticlePediatric surgery international2021

Administration of extracellular vesicles derived from human amniotic fluid stem cells: a new treatment for necrotizing enterocolitis.

Joshua S O'Connell, Carol Lee, Nassim Farhat, Lina Antounians, Augusto Zani, Bo Li, Agostino Pierro

Abstract read
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Article in Pediatric surgery international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Joshua S O'ConnellDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.
Carol LeeDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.
Nassim FarhatDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.
Lina AntouniansDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.
Augusto ZaniDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.
Bo LiDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada.
Agostino PierroDivision of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada. agostino.pierro@sickkids.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeNecrotizing enterocolitis (NEC) is a devastating gastrointestinal disease. Amniotic fluid stem cells (AFSC) improve NEC injury but human translation remains difficult. We aimed to evaluate the use of extracellular vesicles (EV) derived from human AFSC.

methodsHuman AFSC (hAFSC) were cultured according to the protocol (Celprogen Inc., California, U.S.A.). Conditioned medium was obtained, ultra-centrifuged, and EV were suspended in phosphate-buffered saline (PBS). C57BL/6 pups were grouped into: (1) breast-fed (Control, n = 11); (2) NEC + placebo (NEC + PBS; n = 10); and (3) NEC + treatment (NEC + EV; n = 11). NEC was induced post-natal days P5-9 by (A) gavage feeding hyperosmolar formula; (B) hypoxia for 10 min; and (C) lipopolysaccharide. Intra-peritoneal injections of PBS or hAFSC-EV were given on P6-7. All animals were sacrificed on P9 and terminal ileum harvested.

resultshAFSC-EV administration reduced intestinal injury (p = 0.0048), NEC incidence (score ≥ 2), and intestinal inflammation (IL-6 p < 0.0001; TNF-α p < 0.0001). Intestinal stem cell expression (Lgr5 +) and cellular proliferation (Ki67) were enhanced above control levels following hAFSC-EV administration (Lgr5 p = 0.0003; Ki67 p < 0.0001).

conclusionhAFSC-EV administration reduced intestinal NEC injury and inflammation while increasing stem cell expression and cellular proliferation. hAFSC-EV administration may induce similar beneficial effects to exogenous stem cells.

Indexed as

Amniotic FluidAnimalsAnimals, NewbornCell ProliferationDisease Models, AnimalEnterocolitis, NecrotizingExtracellular VesiclesFemaleHumansIleumInfant, NewbornInfant, Newborn, DiseasesInflammationIntestinesMiceMice, Inbred C57BLTumor Necrosis Factor-alphaAmniotic fluid stem cellsExosomesExtracellular vesiclesNecrotizing enterocolitisPediatric surgery

Identifiers

PMID33566163

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.