Evidence map›Paper›PMID 33564926›Full record

ArticleAnalytical and bioanalytical chemistry2021

Studies on the intracellular accumulation process of methotrexate and its correlation with the key protein using an LC-MS/MS method: a novel way to realize prospective individualized medication.

Zhonghua Ouyang, Jing Huang, Yi Ren, Huanhuan Li, Yao Ding, Ke Zhang, Lei Jiang, Peng Yu

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Zhonghua Ouyang *Xiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Jing Huang *Xiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Yi RenXiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Huanhuan LiXiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Yao DingXiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Ke ZhangXiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Lei JiangXiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China.
Peng YuXiangya School of Pharmaceutical Sciences, Central South University, No. 172, Tongzipo Road, Changsha, 410013, Hunan, China. csu_yupeng@126.com.
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-dose methotrexate (HDMTX) combined with leucovorin (LV) is the first-line drug therapy for many kinds of malignant tumors. However, the specific treatment plans, such as dosage and duration of administration, are usually formulated according to the clinician's experience and therapeutic drug monitoring (TDM) of methotrexate in patients' plasma, which are responsible for strong individual differences of drug usage. A large number of studies have shown that methotrexate targets the inside of the cell. The key cytotoxic component is the methotrexate polyglutamates (MTXPGs) in the cell. The concentration of methotrexate in plasma does not reflect the efficacy and side effects well. Based on mass spectrometry technology, we developed and validated an accurate, sensitive, and stable method to quantify the intracellular MTX (MTXPG

Indexed as

AnimalsChemistry, PharmaceuticalChromatography, LiquidDrug Monitoringgamma-Glutamyl HydrolaseKineticsLeucovorinLeukocytes, MononuclearLimit of DetectionMaleMethotrexatePeptidesPeptide SynthasesPolyglutamic AcidQuality ControlRatsfolylpolyglutamate synthetasegamma-Glutamyl HydrolaseLeucovorinMethotrexatemethotrexate polyglutamatePeptidesPeptide SynthasesPolyglutamic AcidIndividualized medicationKey proteinMethotrexateUPLC-MS/MS

Identifiers

PMID33564926
OpenAlexW3128788983

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.