ArticleScientific reports2021
Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- PMAxx dye-assisted colorimetric dual DNAzyme-LAMP (PD-cDDLAMP) for rapid phenotypic antimicrobial susceptibility testing ofMicrobiology spectrum · 2026Article
- Detection of Live Shiga Toxin-ProducingInternational journal of molecular sciences · 2025Article
- Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK System.Methods in molecular biology (Clifton, N.J.) · 2023Article
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Authors and funding
7 authors.
Funding
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Abstract
Spread of drug-resistant bacteria is a serious problem worldwide. We thus designed a new sequence-based protocol that can quickly identify bacterial compositions of clinical samples and their drug-resistance profiles simultaneously. Here we utilized propidium monoazide (PMA) that prohibits DNA amplifications from dead bacteria, and subjected the original and antibiotics-treated samples to 16S rRNA metagenome sequencing. We tested our protocol on bacterial mixtures, and observed that sequencing reads derived from drug-resistant bacteria were significantly increased compared with those from drug-sensitive bacteria when samples were treated by antibiotics. Our protocol is scalable and will be useful for quickly profiling drug-resistant bacteria.
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