ArticleMicrobes and environments2021
Soil Sample Preservation Strategy Affects the Microbial Community Structure.
Article in Microbes and environments, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 45 citations in OpenAlex.
- Assessment of Stability of Preservation Techniques for Strawberry Soil Microbiomes: Exploring the Biotechnological Relevance for Sustainable Agriculture.Microorganisms · 2026Article
- Microbial community characterization in Red Sea-derived samples using a field-deployable DNA extraction system and nanopore sequencing.Environmental microbiome · 2025Article
- Long-term stability of soil spectral libraries with chemical and spectral insights.Scientific reports · 2025Article
- Desiccation as a suitable alternative to cold-storage of phyllosphere samples for DNA-based microbial community analyses.Scientific reports · 2025Article
- Not all is lost: resilience of microbiome samples to freezer failures and long-term storage.mSphere · 2025Article
- Identification of Ancylostoma caninum in Domestic Dogs from Ecuador via Various Techniques.Medical science monitor : international medical journal of experimental and clinical research · 2025Article
- Florida Keys Cassiopea host benthos-like external microbiomes and a gut dominated by Vibrio, Endozoicomonas and Mycoplasma.PloS one · 2025Article
- Effects of continuous cropping on soil metabolomics and rhizosphere bacterial communities inFrontiers in microbiology · 2025Article
- Culturomics- and metagenomics-based insights into the soil microbiome preservation and application for sustainable agriculture.Frontiers in microbiology · 2024Review
- Endophytic and epiphytic metabarcoding reveals fungal communities on cashew phyllosphere in Kenya.PloS one · 2024Article
- Article
- Antarctic Hairgrass Rhizosphere Microbiomes: Microscale Effects Shape Diversity, Structure, and Function.Microbes and environments · 2022Article
- Storage of soil microbiome for application in sustainable agriculture: prospects and challenges.Environmental science and pollution research international · 2022Review
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sample preservation is a critical procedure in any research that relies on molecular tools and is conducted in remote areas. Sample preservation options include low and room temperature storage, which require freezing equipment and specific buffering solutions, respectively. The aim of the present study was to investigate whether DNA/RNA Shield 1x from Zymo Research and DESS (Dimethyl sulfoxide, Ethylenediamine tetraacetic acid, Saturated Salt) solution performed similarly to snap freezing in liquid nitrogen. Soil samples were stored for 1 month in each of the buffers and without any solution at a range of temperatures: -20, +4, and +23°C. All treatments were compared to the "optimal treatment", namely, snap freezing in liquid nitrogen. The quality and quantity of DNA were analyzed, and the microbial community structure was investigated in all samples. The results obtained indicated that the quantity and integrity of DNA was preserved well in all samples; however, the taxonomic distribution was skewed in samples stored without any solution at ambient temperatures, particularly when analyses were performed at lower taxonomic levels. Although both solutions performed equally well, sequencing output and OTU numbers in DESS-treated samples were closer to those snap frozen with liquid nitrogen. Furthermore, DNA/RNA Shield-stored samples performed better for the preservation of rare taxa.
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