ArticleThe Plant cell2021
Specific decorations of 17-hydroxygeranyllinalool diterpene glycosides solve the autotoxicity problem of chemical defense in Nicotiana attenuata.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Drought Stress Mediated Changes in Food Crops: Mechanisms and Remediation Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Preparation and analysis of tobacco glycosides, and the relationship between glycoside aglycones and pyrolysis products: a review.Frontiers in molecular biosciences · 2026Review
- BpWRKY6 regulates insect resistance by affecting jasmonic acid and terpenoid synthesis in Betula platyphylla.Plant biotechnology journal · 2025Article
- Ontogeny and organ-specific steroidal glycoside diversity is associated with differential expression of steroidal glycoside pathway genes in two Solanum dulcamara leaf chemotypes.Plant biology (Stuttgart, Germany) · 2025Article
- Color-dependent defense mechanisms of Quinoa (Chenopodium quinoa Willd.) against Spodoptera exigua: metabolomic and transcriptomic insights.BMC plant biology · 2025Article
- Integrated metabolomic and transcriptomic profiling elucidates the tissue-specific biosynthesis and regulation of flavonoids inFrontiers in plant science · 2025Article
- Engineering Escherichia coli via introduction of the isopentenol utilization pathway to effectively produce geranyllinalool.Microbial cell factories · 2024Article
- Article
- Roles of three cytochrome P450 monooxygenases in triterpene biosynthesis and their potential impact on growth and development.Plant physiology · 2024Article
- OXIDOSQUALENE CYCLASE 1 and 2 influence triterpene biosynthesis and defense in Nicotiana attenuata.Plant physiology · 2024Article
- Exploring the Evolvability of Plant Specialized Metabolism: Uniqueness Out Of Uniformity and Uniqueness Behind Uniformity.Plant & cell physiology · 2023Review
- Functional characterization and structural basis of a reversible glycosyltransferase involves in plant chemical defence.Plant biotechnology journal · 2023Article
- Harmonizing biosynthesis with post-ingestive modifications to understand the ecological functions of plant natural products.Natural product reports · 2022Review
- Into a dilemma of plants: the antagonism between chemical defenses and growth.Plant molecular biology · 2022Review
- The downside of metabolic diversity: Postingestive rearrangements by specialized insects.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Identification and characterization of twoHorticulture research · 2022Article
- Identification and Characterization of a Trillin Rhamnosyltransferase FromFrontiers in plant science · 2021Article
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10 authors.
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Abstract
The native diploid tobacco Nicotiana attenuata produces abundant, potent anti-herbivore defense metabolites known as 17-hydroxygeranyllinalool diterpene glycosides (HGL-DTGs) whose glycosylation and malonylation biosynthetic steps are regulated by jasmonate signaling. To characterize the biosynthetic pathway of HGL-DTGs, we conducted a genome-wide analysis of uridine diphosphate glycosyltransferases (UGTs) and identified 107 family-1 UGT members. The transcript levels of three UGTs were highly correlated with the transcript levels two key HGL-DTG biosynthetic genes: geranylgeranyl diphosphate synthase (NaGGPPS) and geranyllinalool synthase (NaGLS). NaGLS's role in HGL-DTG biosynthesis was confirmed by virus-induced gene silencing. Silencing the Uridine diphosphate (UDP)-rhamnosyltransferase gene UGT91T1 demonstrated its role in the rhamnosylation of HGL-DTGs. In vitro enzyme assays revealed that UGT74P3 and UGT74P4 use UDP-glucose for the glucosylation of 17-hydroxygeranyllinalool (17-HGL) to lyciumoside I. Plants with stable silencing of UGT74P3 and UGT74P5 were severely developmentally deformed, pointing to a phytotoxic effect of the aglycone. The application of synthetic 17-HGL and silencing of the UGTs in HGL-DTG-free plants confirmed this phytotoxic effect. Feeding assays with tobacco hornworm (Manduca sexta) larvae revealed the defensive functions of the glucosylation and rhamnosylation steps in HGL-DTG biosynthesis. Glucosylation of 17-HGL is therefore a critical step that contributes to the resulting metabolites' defensive function and solves the autotoxicity problem of this potent chemical defense.
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