ArticleG3 (Bethesda, Md.)2021
De novo genome assembly of the tobacco hornworm moth (Manduca sexta).
Article in G3 (Bethesda, Md.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Mechanisms of Aristolochic Acid Resistance in Specialist Butterflies and Evolutionary Insights for Potential Protective Pathways.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The role of a viral symbiont in the thermal mismatch of host-parasitoid interactions.The Journal of experimental biology · 2026Article
- High-quality whole genome data ofData in brief · 2025Article
- Article
- A chromosome-level genome assembly of beet webworm, Loxostege sticticalis Linnaeus (Lepidoptera: Pyralidae).Scientific data · 2025Article
- Establishment & characterization of a non-adherent insect cell line for cultivated meat.Scientific reports · 2025Article
- Genomic Monitoring of a Reintroduced Butterfly Uncovers Contrasting Founder Lineage Survival.Evolutionary applications · 2025Article
- Standardized methods for rearing a moth larva, Manduca sexta, in a laboratory setting.PloS one · 2025Article
- The caterpillar Manduca sexta brain shows changes in gene expression and protein abundance correlating with parasitic manipulation of behaviour.Scientific reports · 2024Article
- Chromosome-level genome assembly and annotation of Clanis bilineata tsingtauica Mell (Lepidoptera: Sphingidae).Scientific data · 2024Article
- A long-read draft assembly of the Chinese mantis (Mantodea: Mantidae: Tenodera sinensis) genome reveals patterns of ion channel gain and loss across Arthropoda.G3 (Bethesda, Md.) · 2024Article
- Article
- An enteric ultrastructural surface atlas of the model insectiScience · 2024Article
- High-quality haploid genomes corroborate 29 chromosomes and highly conserved synteny of genes in Hyles hawkmoths (Lepidoptera: Sphingidae).BMC genomics · 2023Article
- A salivary GMC oxidoreductase of Manduca sexta re-arranges the green leaf volatile profile of its host plant.Nature communications · 2023Article
- Article
- A high-quality, long-read genome assembly of the whitelined sphinx moth (Lepidoptera: Sphingidae: Hyles lineata) shows highly conserved melanin synthesis pathway genes.G3 (Bethesda, Md.) · 2023Article
- Genome-wide identification, classification, and expression profiling of serine esterases and other esterase-related proteins in the tobacco hornworm, Manduca sexta.Insect science · 2023Article
- Reference Genome Sequences of the Oriental Armyworm,Insects · 2022Article
- Parallel evolution of opsin visual pigments in hawkmoths by tuning of spectral sensitivities during transition from a nocturnal to a diurnal ecology.The Journal of experimental biology · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The tobacco hornworm, Manduca sexta, is a lepidopteran insect that is used extensively as a model system for studying insect biology, development, neuroscience, and immunity. However, current studies rely on the highly fragmented reference genome Msex_1.0, which was created using now-outdated technologies and is hindered by a variety of deficiencies and inaccuracies. We present a new reference genome for M. sexta, JHU_Msex_v1.0, applying a combination of modern technologies in a de novo assembly to increase continuity, accuracy, and completeness. The assembly is 470 Mb and is ∼20× more continuous than the original assembly, with scaffold N50 > 14 Mb. We annotated the assembly by lifting over existing annotations and supplementing with additional supporting RNA-based data for a total of 25,256 genes. The new reference assembly is accessible in annotated form for public use. We demonstrate that improved continuity of the M. sexta genome improves resequencing studies and benefits future research on M. sexta as a model organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.