ArticlePlant biotechnology journal2021
The OsmiRNA166b-OsHox32 pair regulates mechanical strength of rice plants by modulating cell wall biosynthesis.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- What Do We Know about Barley miRNAs?International journal of molecular sciences · 2022Pooled it
- Genome-Wide Analysis ofPlants (Basel, Switzerland) · 2026Article
- NAC transcription factor GbNTL9 modifies the accumulation and organization of cellulose microfibrils to enhance cotton fiber strength.Journal of advanced research · 2025Article
- Spatiotemporal Transcriptome Profiling Reveals Nutrient Transport Dynamics in Rice Nodes and Roots During Reproductive Development.International journal of molecular sciences · 2025Article
- Modulation of morphogenesis and metabolism by plant cell biomechanics: from model plants to traditional herbs.Horticulture research · 2025Article
- Review
- Knockdown of microRNA390 Enhances Maize Brace Root Growth.International journal of molecular sciences · 2024Article
- The miRNA-mRNA Regulatory Modules ofInternational journal of molecular sciences · 2023Article
- Genome-wide characterization and expression analysis of MYB transcription factors in Chrysanthemum nankingense.BMC plant biology · 2023Article
- Overview of Repressive miRNA Regulation by Short Tandem Target Mimic (STTM): Applications and Impact on Plant Biology.Plants (Basel, Switzerland) · 2023Review
- ZmHOX32 is related to photosynthesis and likely functions in plant architecture of maize.Frontiers in plant science · 2023Article
- Article
- Tapping into the plasticity of plant architecture for increased stress resilience.F1000Research · 2023Review
- AirMeasurer: open-source software to quantify static and dynamic traits derived from multiseason aerial phenotyping to empower genetic mapping studies in rice.The New phytologist · 2022Article
- Cell wall integrity regulation across plant species.Plant molecular biology · 2022Review
- Association mapping uncovers maizeFrontiers in plant science · 2022Article
- Identification of Nitrogen Starvation-Responsive miRNAs to Reveal the miRNA-Mediated Regulatory Network inFrontiers in genetics · 2022Article
- The OsmiRNA166b-OsHox32 pair regulates mechanical strength of rice plants by modulating cell wall biosynthesis.Plant biotechnology journal · 2021Article
- MicroRNA Techniques: Valuable Tools for Agronomic Trait Analyses and Breeding in Rice.Frontiers in plant science · 2021Review
- Genome-Wide Identification of the HD-ZIP III Subfamily in Upland Cotton Reveals the Involvement of GhHB8-5D in the Biosynthesis of Secondary Wall in Fiber and Drought Resistance.Frontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant cell wall provides mechanical strength to support plant growth and development and to determine plant architecture. Cellulose and mixed-linkage glucan (MLG) present in primary cell wall, whereas cellulose, lignin and hemicellulose exist in secondary cell wall. Biosynthesis of the cell wall biopolymers needs the coordinated transcriptional regulation of all the biosynthetic genes. The module of OsmiR166b-OsHox32 regulates expression levels of the genes related to biosynthesis of MLG, cellulose and lignin. Transgenic plants knocking down miR166b (STTM166b) by short tandem target mimic (STTM) technology or overexpressing OsHox32 (OEHox32) showed drooping leaves and brittle culms. Due to accumulation of less lignin and cellulose, the cell wall thickness of STTM166b and OEHox32 plants was reduced when compared to that of wild-type plants. Overexpression of miR166b (OE166b) in rice plants or knocking down of OsHox32 by RNA interference (RNAiHox32) led to increased thickness of cell walls and enhanced mechanical strength of culms. Molecular analyses showed that OsmiR166b-OsHox32 pair regulates cell wall-related gene expression. OsHox32 binds to the promoters of OsCAD2 and OsCESA7 to suppress the expression levels of these two genes. The suppression of OsCAD2 is synergistic when OsHox32 is co-expressed with OSH15 (Oryza sativa homeobox 15). OsHox32 interacts with OSH15, and the START domain of OsHox32, harbouring the miR166b cleavage site, is required for the interaction of these two proteins. Our results demonstrate that OsmiR166b-OsHox32 pair plays important roles not only in plant growth and development but also in plant architecture by regulating the cell wall-related gene expression.
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