ArticlePlant physiology2021
Kinase SnRK1.1 regulates nitrate channel SLAH3 engaged in nitrate-dependent alleviation of ammonium toxicity.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- SlSLAH2 mediates malate exudation and contributes to aluminum tolerance.Nature communications · 2026Article
- Nitrogen use efficiency in crops under salt stress: from molecular networks to intelligent breeding.Frontiers in plant science · 2026Review
- Hydrogen sulfide promotes lateral root formation in peach through persulfidation of SnRK1α kinase.Plant biotechnology journal · 2025Article
- Genome-Wide Investigation ofInternational journal of molecular sciences · 2025Article
- Phenotype Assessment and Putative Mechanisms of Ammonium Toxicity to Plants.International journal of molecular sciences · 2025Review
- Physiological, transcriptomic, and genomic analysis unravels the response of Tatary buckwheat root to high ammonium stress.Frontiers in plant science · 2025Article
- Mechanistic insights into phosphoactivation of SLAC1 in guard cell signaling.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The critical roles of three sugar-related proteins (HXK, SnRK1, TOR) in regulating plant growth and stress responses.Horticulture research · 2024Article
- Article
- Protein Phosphorylation Orchestrates Acclimations of Arabidopsis Plants to Environmental pH.Molecular & cellular proteomics : MCP · 2024Article
- Research progress of the detection and analysis methods of heavy metals in plants.Frontiers in plant science · 2024Review
- The Plasma Membrane Purinoreceptor P2K1/DORN1 Is Essential in Stomatal Closure Evoked by Extracellular Diadenosine Tetraphosphate (ApInternational journal of molecular sciences · 2023Article
- A Soybean Sucrose Non-Fermenting Protein Kinase 1 Gene,International journal of molecular sciences · 2023Article
- The anion channel SLAH3 interacts with potassium channels to regulate nitrogen-potassium homeostasis and the membrane potential in Arabidopsis.The Plant cell · 2023Article
- Recent insights into metabolic and signalling events of directional root growth regulation and its implications for sustainable crop production systems.Frontiers in plant science · 2023Review
- Compartmentalization, a key mechanism controlling the multitasking role of the SnRK1 complex.Journal of experimental botany · 2022Review
- Type one protein phosphatase regulates fixed-carbon starvation-induced autophagy in Arabidopsis.The Plant cell · 2022Article
- Regulatory functions of cellular energy sensor SnRK1 for nitrate signalling through NLP7 repression.Nature plants · 2022Article
- Review
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11 authors.
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Abstract
Nitrate (NO3-) and ammonium (NH4+) are major inorganic nitrogen (N) supplies for plants, but NH4+ as the sole or dominant N source causes growth inhibition in many plants, known as ammonium toxicity. Small amounts of NO3- can significantly mitigate ammonium toxicity, and the anion channel SLAC1 homolog 3 (SLAH3) is involved in this process, but the mechanistic detail of how SLAH3 regulates nitrate-dependent alleviation of ammonium toxicity is still largely unknown. In this study, we identified SnRK1.1, a central regulator involved in energy homeostasis, and various stress responses, as a SLAH3 interactor in Arabidopsis (Arabidopsis thaliana). Our results suggest that SNF1-related protein kinase 1 (SnRK1.1) functions as a negative regulator of SLAH3. Kinase assays indicate SnRK1.1 strongly phosphorylates the C-terminal of SLAH3 at the site S601. Under high-NH4+/low-pH condition, phospho-mimetic and phospho-dead mutations in SLAH3 S601 result in barely rescued phenotypes and fully complemented phenotypes in slah3. Furthermore, SnRK1.1 migrates from cytoplasm to nucleus under high-NH4+/low-pH conditions. The translocation of SnRK1.1 from cytosol to nucleus under high-ammonium stress releases the inhibition on SLAH3, which allows SLAH3-mediated NO3- efflux leading to alleviation of high-NH4+/low-pH stress. Our study reveals that the C-terminal phosphorylation also plays important role in SLAH3 regulation and provides additional insights into nitrate-dependent alleviation of ammonium toxicity in plants.
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