ArticleBone & joint research2021
Exposure to hypergravity during zebrafish development alters cartilage material properties and strain distribution.
Article in Bone & joint research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Hypergravity, Microgravity, and Animal Research.Methods in molecular biology (Clifton, N.J.) · 2026Review
- The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity.Biology open · 2025Article
- Growth and mineralization of fetal mouse long bones under microgravity and daily 1 g gravity exposure.NPJ microgravity · 2024Article
- Treadmill exercise promotes bone tissue recovery in rats subjected to high + Gz loads.Journal of bone and mineral metabolism · 2024Article
- Vibration Rather than Microgravity Affects Bone Metabolism in Adult Zebrafish Scale Model.Cells · 2024Article
- Compressive stress gradients direct mechanoregulation of anisotropic growth in the zebrafish jaw joint.PLoS computational biology · 2024Article
- Hypergravity stimulates mechanical behavior and micro-architecture of tibia in rats.Journal of bone and mineral metabolism · 2024Article
- Growth orientations, rather than heterogeneous growth rates, dominate jaw joint morphogenesis in the larval zebrafish.Journal of anatomy · 2022Article
- GDF11 inhibits abnormal adipogenesis of condylar chondrocytes in temporomandibular joint osteoarthritis.Bone & joint research · 2022Article
- Osteocyte-specific dentin matrix protein 1 : the role of mineralization regulation in low-magnitude high-frequency vibration enhanced osteoporotic fracture healing.Bone & joint research · 2022Article
- Control of the Autophagy Pathway in Osteoarthritis: Key Regulators, Therapeutic Targets and Therapeutic Strategies.International journal of molecular sciences · 2021Review
- Fish Models of Induced Osteoporosis.Frontiers in cell and developmental biology · 2021Review
- Potential of zebrafish as a model to characterise MicroRNA profiles in mechanically mediated joint degeneration.Histochemistry and cell biology · 2020Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
aimsVertebrates have adapted to life on Earth and its constant gravitational field, which exerts load on the body and influences the structure and function of tissues. While the effects of microgravity on muscle and bone homeostasis are well described, with sarcopenia and osteoporosis observed in astronauts returning from space, the effects of shorter exposures to increased gravitational fields are less well characterized. We aimed to test how hypergravity affects early cartilage and skeletal development in a zebrafish model.
methodsWe exposed zebrafish to 3 g and 6 g hypergravity from three to five days post-fertilization, when key events in jaw cartilage morphogenesis occur. Following this exposure, we performed immunostaining along with a range of histological stains and transmission electron microscopy (TEM) to examine cartilage morphology and structure, atomic force microscopy (AFM) and nanoindentation experiments to investigate the cartilage material properties, and finite element modelling to map the pattern of strain and stress in the skeletal rudiments.
resultsWe did not observe changes to larval growth, or morphology of cartilage or muscle. However, we observed altered mechanical properties of jaw cartilages, and in these regions we saw changes to chondrocyte morphology and extracellular matrix (ECM) composition. These areas also correspond to places where strain and stress distribution are predicted to be most different following hypergravity exposure.
conclusionOur results suggest that altered mechanical loading, through hypergravity exposure, affects chondrocyte maturation and ECM components, ultimately leading to changes to cartilage structure and function. Cite this article:
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