Evidence map›Paper›PMID 33558648›Full record

ArticleThe Journal of antibiotics2021

A novel oxazole-containing tetraene compound, JBIR-159, produced by heterologous expression of the cryptic trans-AT type polyketide synthase biosynthetic gene cluster.

Takuya Hashimoto, Junko Hashimoto, Noritaka Kagaya, Takehiro Nishimura, Hikaru Suenaga, Makoto Nishiyama, Tomohisa Kuzuyama, Kazuo Shin-Ya

Abstract read
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In one paragraph

Article in The Journal of antibiotics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Bacterial natural product discovery by heterologous expression.Journal of industrial microbiology & biotechnology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Takuya HashimotoNational Institute of Advanced Industrial Science and Technology, Tokyo, Japan.ORCID http://orcid.org/0000-0002-6261-453X
Junko HashimotoJapan Biological Informatics Consortium (JBIC), Tokyo, Japan.ORCID http://orcid.org/0000-0002-3831-1846
Noritaka KagayaNational Institute of Advanced Industrial Science and Technology, Tokyo, Japan.ORCID http://orcid.org/0000-0002-0622-983X
Takehiro NishimuraTechnology Research Association for Next Generation Natural Products Chemistry, Tokyo, Japan.
Hikaru SuenagaNational Institute of Advanced Industrial Science and Technology, Tokyo, Japan.
Makoto NishiyamaBiotechnology Research Center, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0001-8143-8052
Tomohisa KuzuyamaCollaborative Research Institute for Innovative Microbiology, The University of Tokyo, Tokyo, Japan.
Kazuo Shin-YaNational Institute of Advanced Industrial Science and Technology, Tokyo, Japan. k-shinya@aist.go.jp.ORCID http://orcid.org/0000-0002-4702-0661
National Institute of Advanced Industrial Science and Technology · JPThe University of Tokyo · JPJapan Biological Informatics Consortium · JPSony Computer Science Laboratories · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using genome mining approach, we identified a novel biosynthetic gene cluster containing trans-AT type PKS genes from Streptomyces versipellis 4083-SVS6. A bacterial artificial chromosome (BAC) clone, pKU503JL68_PN1_P10-C12, accommodating the entire biosynthetic gene cluster was obtained from a BAC library. Heterologous expression of the biosynthetic gene cluster in Streptomyces lividans TK23 led to the production of a novel polyene compound, JBIR-159. We report herein the biosynthetic gene cluster for JBIR-159, and the heterologous expression, isolation, structure determination and a brief biological activity.

Indexed as

Chromosomes, Artificial, BacterialCloning, MolecularGene Expression Regulation, BacterialStreptomyces

Identifiers

PMID33558648
OpenAlexW3127658848

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.