ArticleZebrafish2021
TAEL 2.0: An Improved Optogenetic Expression System for Zebrafish.
Article in Zebrafish, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- An optogenetic toolkit for robust activation of FGF, BMP, & Nodal signaling in zebrafish.Developmental biology · 2026Article
- Tunable gene expression in zebrafish using RiboSCALE.bioRxiv : the preprint server for biology · 2026Article
- Endodermal cells use contact inhibition of locomotion to achieve uniform cell dispersal during zebrafish gastrulation.iScience · 2025Article
- MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox for use in vertebrate model systems.Development (Cambridge, England) · 2025Article
- Optogenetic control of Nodal signaling patterns.Development (Cambridge, England) · 2025Article
- Optogenetic Control of Phosphate-Responsive Genes Using Single-Component Fusion Proteins inACS synthetic biology · 2024Article
- Optogenetic control of phosphate-responsive genes using single component fusion proteins inbioRxiv : the preprint server for biology · 2024Article
- Tissue-specific and endogenous protein labeling with split fluorescent proteins.Developmental biology · 2024Article
- Optogenetic control of Nodal signaling patterns.bioRxiv : the preprint server for biology · 2024Article
- Tissue-specific and endogenous protein labeling with split fluorescent proteins.bioRxiv : the preprint server for biology · 2024Article
- Optogenetic Signaling Activation in Zebrafish Embryos.Journal of visualized experiments : JoVE · 2023Article
- A spatiotemporal barrier formed by Follistatin is required for left-right patterning.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Synthetic developmental biology: New tools to deconstruct and rebuild developmental systems.Seminars in cell & developmental biology · 2023Review
- A Single-Component Blue Light-Induced System Based on EL222 inInternational journal of molecular sciences · 2022Article
- Seeing the Light: The Use of Zebrafish for Optogenetic Studies of the Heart.Frontiers in physiology · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Inducible gene expression systems are valuable tools for studying biological processes. We previously developed an optogenetic gene expression system called TAEL that is optimized for use in zebrafish. When illuminated with blue light, TAEL transcription factors dimerize and activate gene expression downstream of the TAEL-responsive C120 promoter. By using light as the inducing agent, the TAEL/C120 system overcomes limitations of traditional inducible expression systems by enabling fine spatial and temporal regulation of gene expression. In this study, we describe ongoing efforts to improve the TAEL/C120 system. We made modifications to both the TAEL transcriptional activator and the C120 regulatory element, collectively referred to as TAEL 2.0. We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system. With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter. We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system. This improved optogenetic expression system will be a broadly useful resource for the zebrafish community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.