Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.

Nicole L Diggins, Lindsey B Crawford, Hillary M Struthers, Lauren M Hook, Igor Landais, Rebecca L Skalsky, Meaghan H Hancock

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Nicole L DigginsVaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.

Lindsey B CrawfordVaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.

Hillary M StruthersVaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.

Lauren M HookVaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.

Igor LandaisVaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.

Rebecca L SkalskyVaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.

Meaghan H HancockVaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA. hancocme@ohsu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level by binding to sites within the 3' untranslated regions of messenger RNA (mRNA) transcripts. The discovery of this completely new mechanism of gene regulation necessitated the development of a variety of techniques to further characterize miRNAs, their expression, and function. In this chapter, we will discuss techniques currently used in the miRNA field to detect, express and inhibit miRNAs, as well as methods used to identify and validate their targets, specifically with respect to the miRNAs encoded by human cytomegalovirus.

Indexed as

3' Untranslated RegionsBlotting, NorthernCytomegalovirusGene ExpressionGene Expression Regulation, ViralHumansImmunoprecipitationMicroRNAsRNA, Messenger3' Untranslated RegionsMicroRNAsRNA, MessengerHuman cytomegalovirus (HCMV)Locked nucleic acids (LNA)Luciferase assaymicroRNA (miRNA)Northern BlotPhotoactivatable ribonucleoside-enhanced cross-linking and Immunoprecipitation (PAR CLIP)RISC-immunoprecipitation (RISC-IP)Stem-loop real-time PCR

Identifiers

PMID33555594

DOI10.1126/science.1178178

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.