ArticleGenes & genomics2021
Genome wide methylation analysis to uncover genes related to recurrent pregnancy loss.
Article in Genes & genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 51 citations in OpenAlex.
- Epigenetic regulation of HLA-G : implications for recurrent pregnancy loss.Molecular biology reports · 2026Article
- Epigenetics of maternal-fetal interface immune microenvironment and placental related pregnancy complications.Frontiers in immunology · 2025Review
- Dysregulation of CREB5 Impairs Decidualization and Maternal-Fetal Interactions by Inhibiting Autophagy in Recurrent Spontaneous Abortion.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- The role of DNA methylation in placental development and its implications for preeclampsia.Frontiers in cell and developmental biology · 2024Review
- DNA methylation abnormalities induced by advanced maternal age in villi prime a high-risk state for spontaneous abortion.Clinical epigenetics · 2023Article
- Integrated single-cell RNA-seq and DNA methylation reveal the effects of air pollution in patients with recurrent spontaneous abortion.Clinical epigenetics · 2022Article
- Review
- Association Study between Mucin 4 (Genes · 2022Article
- Association of Polymorphisms in Plasminogen Activator Inhibitor-1 (Journal of personalized medicine · 2021Article
- DNA Methylation and Recurrent Pregnancy Loss: A Mysterious Compass?Frontiers in immunology · 2021Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecurrent pregnancy loss (RPL) refers to two or more consecutive spontaneous abortion before 24 weeks of gestation, representing 1% of couples of childbearing age. Epigenetic factors including dysregulation of DNA methylation of some genes may play a role in RPL.
objectiveTo identify RPL related genes modulated by DNA methylation expressed in decidua and blood.
methodsThree decidua samples each from RPL patients and normal controls were recruited to perform genome-wide bisulfite sequencing (GWBS) and transcriptome sequencing. Based on the above results, 22.52 kb of differential methylation regions (DMRs) from 17 genes were verified by bisulfite sequencing PCR at specific region (Hi-MethylSeq) in another 15 decidua (7RPL vs. 8 Controls) and 13 blood (5RPL vs. 8 Controls) samples.
results23 genes showed significantly differential cytosine methylation status and distinct expression level between PRL patients and healthy controls synergistically. Three signaling pathways were found to be shared between genes with both hypomethylated differential methylation regions (DMR) and upregulated differential gene expression (DGE). The results from Hi-MethylSeq showed that the hypermethylation of SGK1 in both blood and decidua samples in RPL patients, which was consistent to its lower expression in endometrium reported earlier. SGK3 and CREB5 also showed modulated methylation level in RPL decidua.
conclusionOur finding supported that aberrant methylation of SGK1 and CREB5 could be a cause of the dysregulation of these gens in the endometrium, which is one of cause of reproductive failure. The function of SGK3 in reproduction system deserves further investigation.
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