Evidence map›Paper›PMID 33554930›Full record

ArticleJournal of vestibular research : equilibrium & orientation2021

Functional cooperation between two otoconial proteins Oc90 and Nox3.

Yinfang Xu, Liping Yang, Xing Zhao, Yan Zhang, Timothy A Jones, Sherri M Jones, Yunxia Wang Lundberg

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of vestibular research : equilibrium & orientation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. NADPH Oxidase 3: Beyond the Inner Ear.Antioxidants (Basel, Switzerland) · 2024
    Review
  3. Advances in otolith-related protein research.Frontiers in neuroscience · 2022
    Review
  4. Mechanisms of otoconia and otolith development.Developmental dynamics : an official publication of the American Association of Anatomists · 2015
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Yinfang XuVestibular Genetics Laboratory, Boys Town National Research Hospital, Omaha, NE, USA.
Liping YangVestibular Genetics Laboratory, Boys Town National Research Hospital, Omaha, NE, USA.
Xing ZhaoVestibular Genetics Laboratory, Boys Town National Research Hospital, Omaha, NE, USA.
Yan ZhangVestibular Genetics Laboratory, Boys Town National Research Hospital, Omaha, NE, USA.
Timothy A JonesDepartment of Special Education and Communication Disorders, University of Nebraska-Lincoln, Lincoln, NE, USA.
Sherri M JonesDepartment of Special Education and Communication Disorders, University of Nebraska-Lincoln, Lincoln, NE, USA.
Yunxia Wang LundbergVestibular Genetics Laboratory, Boys Town National Research Hospital, Omaha, NE, USA.
Boys Town · USBoys Town National Research Hospital · USUniversity of Nebraska–Lincoln · US

Funding

OTOCONIA DEVELOPMENT AND MAINTENANCER01DC008603 · NIDCD · FATHER FLANAGAN'S BOYS' HOME · PI LUNDBERG, YUNXIA WANG · 2007 to 2011
$1.7M
GENETIC AND GENOMIC CONTRIBUTION TO BALANCE AND HEARING PROBLEMS IN ADULTSK18DC014748 · NIDCD · FATHER FLANAGAN'S BOYS' HOME · PI LUNDBERG, YUNXIA WANG · 2016 to 2017
$352k
NIDCD NIH HHS K18 DC014748NIDCD NIH HHS R01 DC008603
6 · The paper itself

Abstract

backgroundOtoconia-related vertigo and balance deficits are common in humans, but the molecular etiology is unknown at present.

objectiveIn order to study mechanisms of otoconia formation and maintenance, we have investigated whether otoconin-90 (Oc90), the predominant otoconial constituent protein, and the NADPH oxidase Nox3, an essential regulatory protein for otoconia formation, are functionally interlinked.

methodsWe performed balance behavioral, electrophysiological, morphological and molecular cellular analyses.

resultsDouble heterozygous mutant mice for Oc90 and Nox3 show severe imbalance, albeit less profound than double null mutants. In contrast, single heterozygous mutant mice have normal balance. Double heterozygous mice have otoconia defects and double null mice have no otoconia. In addition, some hair bundles in the latter mice go through accelerated degeneration. In vitro calcification analysis in cells stably expressing these proteins singly and doubly shows much more intense calcification in the double transfectants.

conclusionsOc90 and Nox3 augment each other's function, which is not only critical for otoconia formation but also for hair bundle maintenance.

Indexed as

Extracellular Matrix ProteinsNADPH OxidasesOtolithic MembraneAnimalsCalcium-Binding ProteinsMiceVertigoCalcium-Binding ProteinsExtracellular Matrix ProteinsNADPH OxidasesNox3 protein, mouseOc90 protein, mousebalancecalcificationhair bundleNox3Oc90

Identifiers

PMID33554930
PMCPMC8989612
OpenAlexW3126224277

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.