ArticleSTAR protocols2021
Imaging the early zebrafish embryo centrosomes following injection of small-molecule inhibitors to understand spindle formation.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 12 citations in OpenAlex.
- Functionally Essential and Structurally Diverse: Insights into the zebrafish Left-Right Organizer's Cilia via Optogenetic IFT88 Perturbation and Volume Electron Microscopy.bioRxiv : the preprint server for biology · 2025Article
- Specific mitotic events drive left-right organizer development.Development (Cambridge, England) · 2025Article
- Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development.PLoS genetics · 2023Article
- A minimally invasive fin scratching protocol for fast genotyping and early selection of zebrafish embryos.Scientific reports · 2022Article
- Pericentriolar matrix (PCM) integrity relies on cenexin and polo-like kinase (PLK)1.Molecular biology of the cell · 2022Article
- Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo.Life science alliance · 2022Article
- Optogenetic axon guidance in embryonic zebrafish.STAR protocols · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
During the earliest division stages, zebrafish embryos have large cells that divide rapidly and synchronously to create a cellular layer on top of the yolk. Here, we describe a protocol for monitoring spindle dynamics during these early embryonic divisions. We outline techniques for injecting zebrafish embryos with small-molecule inhibitors toward polo-like kinases, preparing and mounting embryos for three-dimensional imaging using confocal microscopy. These techniques are used to understand how the early zebrafish embryo's centrosome constructs the mitotic spindle. For complete details on the use and execution of this protocol, please refer to Rathbun et al. (2020).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.