Evidence map›Paper›PMID 33552748›Full record

ArticleApplications in plant sciences2021

A target enrichment probe set for resolving the flagellate land plant tree of life.

Jesse W Breinholt, Sarah B Carey, George P Tiley, E Christine Davis, Lorena Endara, Stuart F McDaniel, Leandro G Neves, Emily B Sessa, Matt von Konrat, Sahut Chantanaorrapint and 14 more

Abstract read
In one paragraph

Article in Applications in plant sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Guideline
  2. Article
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  5. Article
  6. Phylogenetic Insights from a NovelPlants (Basel, Switzerland) · 2025
    Article
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  12. Unveiling the diversification and dispersal of theFrontiers in plant science · 2024
    Article
  13. PhytoKeys · 2024
    Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Systematics and Plastome Evolution in Schizaeaceae.Frontiers in plant science · 2022
    Article
  20. An open and continuously updated fern tree of life.Frontiers in plant science · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Jesse W BreinholtRAPiD Genomics Gainesville Florida USA.ORCID https://orcid.org/0000-0002-3867-2430
Sarah B CareyDepartment of Biology University of Florida Gainesville Florida USA.ORCID https://orcid.org/0000-0002-6431-0660
George P TileyDepartment of Biology University of Florida Gainesville Florida USA.ORCID https://orcid.org/0000-0003-0053-0207
E Christine DavisDepartment of Biology University of Florida Gainesville Florida USA.
Lorena EndaraDepartment of Biology University of Florida Gainesville Florida USA.ORCID https://orcid.org/0000-0003-2834-7412
Stuart F McDanielDepartment of Biology University of Florida Gainesville Florida USA.ORCID https://orcid.org/0000-0002-5435-7377
Leandro G NevesRAPiD Genomics Gainesville Florida USA.
Emily B SessaDepartment of Biology University of Florida Gainesville Florida USA.ORCID https://orcid.org/0000-0002-6496-5536
Matt von KonratDepartment of Research and Education The Field Museum Chicago Illinois USA.
Sahut ChantanaorrapintDepartment of Biology Faculty of Science Prince of Songkla University Songkhla Thailand.ORCID https://orcid.org/0000-0002-9739-0994
Susan FawcettPringle Herbarium Department of Plant Biology University of Vermont Burlington Vermont USA.ORCID https://orcid.org/0000-0001-5021-1779
Stefanie M Ickert-BondDepartment of Wildlife and Biology and UA Museum of the North University of Alaska Fairbanks Fairbanks Alaska USA.ORCID https://orcid.org/0000-0001-8198-8898
Paulo H LabiakDepartamento de Botânica Universidade Federal do Paraná Curitiba Paraná Brazil.ORCID https://orcid.org/0000-0002-5239-2048
Juan LarraínInstituto de Biología Pontificia Universidad Católica de Valparaíso Valparaíso Chile.ORCID https://orcid.org/0000-0002-9423-6561
Marcus LehnertDepartment of Geobotany and Botanical Garden Herbarium, Martin Luther University Halle-Wittenberg Halle Germany.ORCID https://orcid.org/0000-0002-7202-7734
Lily R LewisDepartment of Biology University of Florida Gainesville Florida USA.
Nathalie S NagalingumCalifornia Academy of Sciences San Francisco California USA.ORCID https://orcid.org/0000-0002-0221-9650
Nikisha PatelDepartment of Ecology and Evolutionary Biology University of Connecticut Storrs Connecticut USA.ORCID https://orcid.org/0000-0002-3504-7314
Stefan A RensingFaculty of Biology University of Marburg Marburg Germany.
Weston TestoDepartment of Biology University of Florida Gainesville Florida USA.ORCID https://orcid.org/0000-0003-3194-5763
Alejandra VascoBotanical Research Institute of Texas Fort Worth Texas USA.ORCID https://orcid.org/0000-0002-6242-4760
Juan Carlos VillarrealDepartment of Biology Laval University Québec City Québec Canada.ORCID https://orcid.org/0000-0002-0770-1446
Evelyn Webb WilliamsChicago Botanic Garden Glencoe Illinois USA.ORCID https://orcid.org/0000-0003-3301-716X
J Gordon BurleighDepartment of Biology University of Florida Gainesville Florida USA.ORCID https://orcid.org/0000-0001-8120-5136

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

premiseNew sequencing technologies facilitate the generation of large-scale molecular data sets for constructing the plant tree of life. We describe a new probe set for target enrichment sequencing to generate nuclear sequence data to build phylogenetic trees with any flagellate land plants, including hornworts, liverworts, mosses, lycophytes, ferns, and all gymnosperms.

methodsWe leveraged existing transcriptome and genome sequence data to design the GoFlag 451 probes, a set of 56,989 probes for target enrichment sequencing of 451 exons that are found in 248 single-copy or low-copy nuclear genes across flagellate plant lineages.

resultsOur results indicate that target enrichment using the GoFlag451 probe set can provide large nuclear data sets that can be used to resolve relationships among both distantly and closely related taxa across the flagellate land plants. We also describe the GoFlag 408 probes, an optimized probe set covering 408 of the 451 exons from the GoFlag 451 probe set that is commercialized by RAPiD Genomics.

conclusionsA target enrichment approach using the new probe set provides a relatively low-cost solution to obtain large-scale nuclear sequence data for inferring phylogenetic relationships across flagellate land plants.

Indexed as

flagellate plantsnext‐generation sequencingnuclear lociphylogenomicstarget enrichment

Identifiers

PMID33552748
PMCPMC7845764

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.