Evidence map›Paper›PMID 33552733›Full record

ArticlePeerJ2021

Physical activity modulates mononuclear phagocytes in mammary tissue and inhibits tumor growth in mice.

Donald M Lamkin, Karen P Bradshaw, Janice Chang, Ma'ayan Epstein, Jack Gomberg, Krupa P Prajapati, Veronica H Soliman, Thezia Sylviana, Yinnie Wong, Kouki Morizono and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Donald M LamkinNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Karen P BradshawNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Janice ChangNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Ma'ayan EpsteinNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Jack GombergNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Krupa P PrajapatiNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Veronica H SolimanNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Thezia SylvianaNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Yinnie WongNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Kouki MorizonoDivison of Hematology-Oncology, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA, United States of America.
Erica K SloanNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Steve W ColeNorman Cousins Center for PNI, Semel Institute for Neuroscience, University of California, Los Angeles, CA, United States of America.
Black AIDS Institute · USMonash University · AUNeurobehavioral Systems · USStanford University · USUniversity of California, Los Angeles · US

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Samuel Wheeler French · 1985 to 2026
$134.5M
Social regulation of pro-inflammatory monocytesR01AG043404 · NIA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI COLE, STEVE W · 2014 to 2018
$1.6M
Translational Research of Biobehavioral Mechanisms in Cancer ControlK07CA188237 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI LAMKIN, DONALD MICHAEL · 2015 to 2019
$766k
NCI NIH HHS K07 CA188237NCI NIH HHS P30 CA016042NIA NIH HHS R01 AG043404
6 · The paper itself

Abstract

The risk for breast cancer is significantly reduced in persons who engage in greater amounts of physical activity, and greater physical activity before or after diagnosis associates with reduced disease-specific mortality. Previous mechanistic studies indicate that components of innate immunity can mediate an inhibitory effect of physical activity on several types of tumor. However, in breast cancer specifically, the myeloid compartment of innate immunity is thought to exhibit high propensity for an immunosuppressive role that obstructs anti-tumor immunity. Thus, we tested the notion that greater physical activity alters mononuclear phagocytes in mammary tissue when inhibiting nascent tumor in a murine model of breast cancer. To model greater physical activity, we placed an angled running wheel in each mouse's home cage for two weeks before tumor engraftment with EO771 mammary cancer cells that express luciferase for bioluminescent detection. Fully immunocompetent mice and mice with compromised adaptive immunity showed significantly less mammary tumor signal when given access to running wheels, although the effect size was smaller in this latter group. To investigate the role of the myeloid compartment, mononuclear phagocytes were ablated by systemic injection of clodronate liposomes at 24 h before tumor engraftment and again at the time of tumor engraftment, and this treatment reversed the inhibition in wheel running mice. However, clodronate also inhibited mammary tumor signal in sedentary mice, in conjunction with an expected decrease in gene and protein expression of the myeloid antigen, F4/80 (Adgre1), in mammary tissue. Whole transcriptome digital cytometry with CIBERSORTx was used to analyze myeloid cell populations in mammary tissue following voluntary wheel running and clodronate treatment, and this approach found significant changes in macrophage and monocyte populations. In exploratory analyses, whole transcriptome composite scores for monocytic myeloid-derived suppressor cell (M-MDSC), macrophage lactate timer, and inflammation resolution gene expression programs were significantly altered. Altogether, the results support the hypothesis that physical activity inhibits nascent mammary tumor growth by enhancing the anti-tumor potential of mononuclear phagocytes in mammary tissue.

Indexed as

Breast cancerExercise oncologyInnate immunityMacrophagesMonocytesMyeloid cellsTranscriptomicsTumor immunologyVoluntary wheel running

Identifiers

PMID33552733
PMCPMC7821756
OpenAlexW3123503922

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.