Evidence map›Paper›PMID 33551454›Full record

ArticleElectrochimica acta2018

A graphene aptasensor for biomarker detection in human serum.

Xuejun Wang, Yibo Zhu, Timothy R Olsen, Na Sun, Wenjun Zhang, Renjun Pei, Qiao Lin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Electrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 61 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Thiol-based chemically modified carbon screen-printed electrode for simultaneous quantification of trace level Pb(II) and Cd(II).Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024
    Article
  5. Aptameric Metal-Organic Framework Nanobiosensor.ACS applied nano materials · 2024
    Article
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  7. Review
  8. Article
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  12. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Xuejun WangDepartment of Mechanical Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Yibo ZhuDepartment of Mechanical Engineering, Columbia University, New York, NY 10027, USA.
Timothy R OlsenDepartment of Mechanical Engineering, Columbia University, New York, NY 10027, USA.
Na SunSuzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China.
Wenjun ZhangDepartment of Mechanical Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Renjun PeiSuzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, Jiangsu, 215123, China.
Qiao LinDepartment of Mechanical Engineering, Columbia University, New York, NY 10027, USA.
Columbia University · USChinese Academy of Sciences · CNEast China University of Science and Technology · CN

Funding

Validating Rapid Microfluidic Isolation of Personalized Aptamers for Monitoring Minimal Residual Disease in Multiple MyelomaR33CA196470 · NCI · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI LIN, QIAO · 2016 to 2018
$1.2M
NCI NIH HHS R33 CA196470
6 · The paper itself

Abstract

This paper presents an affinity graphene nanosensor for detection of biomarkers in undiluted and non-desalted human serum. The affinity nanosensor is a field-effect transistor in which graphene serves as the conducting channel. The graphene surface is sequentially functionalized with a nanolayer of the polymer polyethylene glycol (PEG) and a biomarker-specific aptamer. The aptamer is able to specifically bind with and capture unlabeled biomarkers in serum. A captured biomarker induces a change in the electric conductivity of the graphene, which is measured in a buffer of optimally chosen ionic strength to determine the biomarker concentration. The PEG layer effectively rejects nonspecific adsorption of background molecules in serum while still allowing the aptamer to be readily accessible to serum-borne biomarkers and increases the effective Debye screening length on the graphene surface. Thus, the aptamer-biomarker binding sensitively changes the graphene conductivity, thereby achieving specific and label-free detection of biomarkers with high sensitivity and without the need to dilute or desalt the serum. Experimental results demonstrate that the graphene nanosensor is capable of specifically capturing human immunoglobulin E (IgE), used as a representative biomarker, in human serum in the concentration range of 50 pM-250 nM, with a resolution of 14.5 pM and a limit of detection of 47 pM.

Indexed as

Affinity sensingBiomarker detectionGrapheneHuman serumNonspecific binding

Identifiers

PMID33551454
PMCPMC7861490
OpenAlexW2890502943

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.