Evidence map›Paper›PMID 33547973›Full record

ArticleRice (New York, N.Y.)2021

Efficient Gene Stacking in Rice Using the GAANTRY System.

Leyla T Hathwaik, James Horstman, James G Thomson, Roger Thilmony

Abstract read
In one paragraph

Article in Rice (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Metabolic engineering inaBIOTECH · 2025
    Review
  2. Synthetic crassulacean acid metabolism (SynCAM) for improving water-use efficiency in plants.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  3. Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025
    Review
  4. Review
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Leyla T HathwaikUnited States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research Unit, Albany, CA, 94710, USA.
James HorstmanUnited States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research Unit, Albany, CA, 94710, USA.
James G ThomsonUnited States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research Unit, Albany, CA, 94710, USA.
Roger ThilmonyUnited States Department of Agriculture-Agriculture Research Service, Western Regional Research Center, Crop Improvement and Genetics Research Unit, Albany, CA, 94710, USA. Roger.Thilmony@usda.gov.ORCID http://orcid.org/0000-0002-6591-6699

Funding

Agricultural Research Service 2030-21220-002-00-D
6 · The paper itself

Abstract

Genetic engineering of rice provides a means for improving rice grain quality and yield, and the introduction and expression of multiple genes can produce new traits that would otherwise be difficult to obtain through conventional breeding. GAANTRY (Gene Assembly in Agrobacterium by Nucleic acid Transfer using Recombinase technologY) was previously shown to be a precise and robust system to stably stack ten genes (28 kilobases (kb)) within an Agrobacterium virulence plasmid Transfer-DNA (T-DNA) and obtain high-quality Arabidopsis and potato transgenic events. To determine whether the GAANTRY system can be used to engineer a monocotyledonous crop, two new T-DNA constructs, carrying five (16.9 kb) or eleven (37.4 kb) cargo sequences were assembled and transformed into rice. Characterization of 53 independent transgenic events demonstrated that more than 50% of the plants carried all of the desired cargo sequences and exhibited the introduced traits. Additionally, more than 18% of the lines were high-quality events containing a single copy of the introduced transgenes and were free of sequences from outside of the T-DNA. Therefore, GAANTRY provides a simple, precise and versatile tool for transgene stacking in rice and potentially other cereal grain crops.

Indexed as

AgrobacteriumGene stackingGenetic engineeringOryza sativaSite-specific recombinase

Identifiers

PMID33547973
PMCPMC7867672

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.