ArticleScientific reports2021
Hybridisation capture allows DNA damage analysis of ancient marine eukaryotes.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 63 citations in OpenAlex.
- A Roadmap for Using Hybridisation Capture-Based Target Enrichment of Ancient Environmental DNA in Palaeoecology.Molecular ecology resources · 2026Review
- How low can you go? Establishing detection limits for rare eukaryotes in Southern Ocean sedimentary ancient DNA.Bioinformatics advances · 2026Article
- Continuity of eastern Beringian megafauna phylogenetic diversity following deposition of the Late Pleistocene Dawson tephra.Arctic, antarctic, and alpine research · 2026Article
- Progress, challenges and future directions in marine organic-walled dinoflagellate cyst research: New insights from an international workshop.Marine micropaleontology · 2025Article
- Using sedimentary ancient DNA in coastal and marine contexts to explore past human-environmental interactions in Australia.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- Recovering sedimentary ancient DNA of harmful dinoflagellates accumulated over the last 9000 years off Eastern Tasmania, Australia.ISME communications · 2024Article
- Marine ecosystem shifts with deglacial sea-ice loss inferred from ancient DNA shotgun sequencing.Nature communications · 2023Article
- Early human impact on lake cyanobacteria revealed by a Holocene record of sedimentary ancient DNA.Communications biology · 2023Article
- Review
- Ancient marine sediment DNA reveals diatom transition in Antarctica.Nature communications · 2022Article
- Millennia-old coral holobiont DNA provides insight into future adaptive trajectories.Molecular ecology · 2022Article
- Recovery of chloroplast genomes from medieval millet grains excavated from the Areni-1 cave in southern Armenia.Scientific reports · 2022Article
- The untapped potential of macrofossils in ancient plant DNA research.The New phytologist · 2022Article
- Environmental paleomicrobiology: using DNA preserved in aquatic sediments to its full potential.Environmental microbiology · 2022Review
- Patterns of eukaryotic diversity from the surface to the deep-ocean sediment.Science advances · 2022Article
- Article
- Healthier Communities of Phytoplankton and Bacteria Achieved via the Application of Modified Clay in Shrimp Aquaculture Ponds.International journal of environmental research and public health · 2021Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marine sedimentary ancient DNA (sedaDNA) is increasingly used to study past ocean ecosystems, however, studies have been severely limited by the very low amounts of DNA preserved in the subseafloor, and the lack of bioinformatic tools to authenticate sedaDNA in metagenomic data. We applied a hybridisation capture 'baits' technique to target marine eukaryote sedaDNA (specifically, phyto- and zooplankton, 'Planktonbaits1'; and harmful algal bloom taxa, 'HABbaits1'), which resulted in up to 4- and 9-fold increases, respectively, in the relative abundance of eukaryotes compared to shotgun sequencing. We further used the bioinformatic tool 'HOPS' to authenticate the sedaDNA component, establishing a new proxy to assess sedaDNA authenticity, "% eukaryote sedaDNA damage", that is positively correlated with subseafloor depth. We used this proxy to report the first-ever DNA damage profiles from a marine phytoplankton species, the ubiquitous coccolithophore Emiliania huxleyi. Our approach opens new avenues for the detailed investigation of long-term change and evolution of marine eukaryotes over geological timescales.
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