Evidence map›Paper›PMID 33546406›Full record

ArticleGenes2021

Evaluation of OpenArray™ as a Genotyping Method for Forensic DNA Phenotyping and Human Identification.

Michele Ragazzo, Giulio Puleri, Valeria Errichiello, Laura Manzo, Laura Luzzi, Saverio Potenza, Claudia Strafella, Cristina Peconi, Fabio Nicastro, Valerio Caputo and 1 more

Open access · goldAbstract read
In one paragraph

Article in Genes, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Michele RagazzoDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.
Giulio PuleriDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.
Valeria ErrichielloDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.
Laura ManzoDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.
Laura LuzziDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.ORCID 0000-0001-6137-9366
Saverio PotenzaDepartment of Biomedicine and Prevention, Section of Legal Medicine, Social Security and Forensic Toxicology, University of Rome Tor Vergata, 00133 Rome, Italy.ORCID 0000-0002-1671-779X
Claudia StrafellaDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.ORCID 0000-0003-1334-0920
Cristina PeconiGenomic Medicine Laboratory UILDM, IRCCS Santa Lucia Foundation, 00179 Rome, Italy.
Fabio NicastroAustech, 00153 Rome, Italy.ORCID 0000-0003-3862-4890
Valerio CaputoDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.ORCID 0000-0002-3503-3318
Emiliano GiardinaDepartment of Biomedicine and Prevention, Tor Vergata University of Rome, 00133 Rome, Italy.
University of Rome Tor Vergata · ITFondazione Santa Lucia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A custom plate of OpenArray™ technology was evaluated to test 60 single-nucleotide polymorphisms (SNPs) validated for the prediction of eye color, hair color, and skin pigmentation, and for personal identification. The SNPs were selected from already validated subsets (Hirisplex-s, Precision ID Identity SNP Panel, and ForenSeq DNA Signature Prep Kit). The concordance rate and call rate for every SNP were calculated by analyzing 314 sequenced DNA samples. The sensitivity of the assay was assessed by preparing a dilution series of 10.0, 5.0, 1.0, and 0.5 ng. The OpenArray™ platform obtained an average call rate of 96.9% and a concordance rate near 99.8%. Sensitivity testing performed on serial dilutions demonstrated that a sample with 0.5 ng of total input DNA can be correctly typed. The profiles of the 19 SNPs selected for human identification reached a random match probability (RMP) of, on average, 10

Indexed as

Genotyping TechniquesDNA FingerprintingEye ColorForensic AnthropologyForensic GeneticsGenotypeHair ColorHigh-Throughput Nucleotide SequencingHumansPolymorphism, Single NucleotideSkin Pigmentationforensic DNA phenotypingforensic DNA typingOpenArray™ systemTaqMan SNP genotyping

Identifiers

PMID33546406
PMCPMC7913479
OpenAlexW3128946181

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.