ArticleJournal of structural biology2021
Measuring collagen fibril diameter with differential interference contrast microscopy.
Article in Journal of structural biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Bone-like Collagen Matrices Through Rapid Intrafibrillar Mineralisation.Journal of functional biomaterials · 2026Article
- Collagen fibril diameter quantification using interference confocal reflectance microscopy.Biomedical optics express · 2026Article
- Nanofibrous Platforms for Advanced Wound Therapeutics: Mechanisms, Materials, and Clinical Horizons.Macromolecular bioscience · 2026Review
- Enhancing liver fibrosis measurement: Deep learning and uncertainty analysis across multi-center cohorts.Journal of pathology informatics · 2026Article
- Fibrillar Bundles as Fibrous Filler Materials for Attaining Cell Anisotropy in Bioprinting.Advanced healthcare materials · 2026Article
- On-glass Interference Confocal Reflectance Microscopy for Collagen Fibril Diameter Measurement.Proceedings of SPIE--the International Society for Optical Engineering · 2026Article
- Collagen Mechanics.Sub-cellular biochemistry · 2026Review
- Quantifying cell traction forces at the single-fiber scale in 3D: An approach based on deformable photopolymerized fiber arrays.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Electrospun Polymer Fiber Mats for Persulfide Prodrug Delivery.Biomacromolecules · 2025Article
- Geometric characteristics of stromal collagen fibres in breast cancer using differential interference contrast microscopy.Journal of microscopy · 2025Article
- Interference Confocal Reflectance Microscopy for Collagen Fibril Diameter Measurement.Proceedings of SPIE--the International Society for Optical Engineering · 2025Article
- Augmentation of Tendon and Ligament Repair with Fiber-Reinforced Hydrogel Composites.Advanced healthcare materials · 2024Review
- Dendritic cell force-migration coupling on aligned fiber networks.Biophysical journal · 2024Article
- Synthetic Extracellular Matrix of Polyvinyl Alcohol Nanofibers for Three-Dimensional Cell Culture.Journal of functional biomaterials · 2024Article
- Photo-responsive decellularized small intestine submucosa hydrogels.Advanced functional materials · 2024Article
- Magnetic Alignment of Collagen: Principles, Methods, Applications, and Fiber Alignment Analyses.Tissue engineering. Part B, Reviews · 2024Review
- Article
- Modeling the extracellular matrix in cell migration and morphogenesis: a guide for the curious biologist.Frontiers in cell and developmental biology · 2024Review
- Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons.Biomaterials and biosystems · 2023Article
- Modeling collagen fibril self-assembly from extracellular medium in embryonic tendon.Biophysical journal · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Collagen fibrils, linear arrangements of collagen monomers, 20-500 nm in diameter, comprising hundreds of molecules in their cross-section, are the fundamental structural unit in a variety of load-bearing tissues such as tendons, ligaments, skin, cornea, and bone. These fibrils often assemble into more complex structures, providing mechanical stability, strength, or toughness to the host tissue. Unfortunately, there is little information available on individual fibril dynamics, mechanics, growth, aggregation and remodeling because they are difficult to image using visible light as a probe. The principle quantity of interest is the fibril diameter, which is difficult to extract accurately, dynamically, in situ and non-destructively. An optical method, differential interference contrast (DIC) microscopy has been used to visualize dynamic structures that are as small as microtubules (25 nm diameter) and has been shown to be sensitive to the size of objects smaller than the wavelength of light. In this investigation, we take advantage of DIC microscopy's ability to report dimensions of nanometer scale objects to generate a curve that relates collagen diameter to DIC edge intensity shift (DIC-EIS). We further calibrate the curve using electron microscopy and demonstrate a linear correlation between fibril diameter and the DIC-EIS. Using a non-oil immersion, 40x objective (NA 0.6), collagen fibril diameters between ~100 nm to ~ 300 nm could be obtained with ±11 and ±4 nm accuracy for dehydrated and hydrated fibrils, respectively. This simple, nondestructive, label free method should advance our ability to directly examine fibril dynamics under experimental conditions that are physiologically relevant.
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Registered trials
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