Evidence map›Paper›PMID 33544965›Full record

ArticleJournal of applied microbiology2021

Survival of Staphylococcus aureus on sampling swabs stored at different temperatures.

D Panisello Yagüe, J Mihaljevic, M Mbegbu, C V Wood, C Hepp, S Kyman, H Hornstra, R Trotter, E Cope, T Pearson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of applied microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

D Panisello YagüePathogen & Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
J MihaljevicSchool of Informatics, Computing, and Cyber Systems, Northern Arizona University, Flagstaff, AZ, USA.
M MbegbuPathogen & Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
C V WoodPathogen & Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
C HeppSchool of Informatics, Computing, and Cyber Systems, Northern Arizona University, Flagstaff, AZ, USA.
S KymanPathogen & Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
H HornstraPathogen & Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
R TrotterDepartment of Anthropology, Northern Arizona University, Flagstaff, AZ, USA.
E CopePathogen & Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
T PearsonPathogen & Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.ORCID https://orcid.org/0000-0003-3696-041X

Funding

SSCIMA: Integrating Analysis of Socio-economic Sub-population Dynamics to Improve Spatial Models of Infectious DiseaseU54MD012388 · NIMHD · NORTHERN ARIZONA UNIVERSITY · PI Julie A. Baldwin · 2017 to 2026
$49.7M
Are Minority Health Disparities in MRSA/MSSA Infections Related to Carriage and Social Relationships?R15AI156771 · NIAID · NORTHERN ARIZONA UNIVERSITY · PI PEARSON, TALIMA ROSS · 2021 to 2021
$456k
National Institute of Allergy and Infectious Diseases R15AI156771NIAID NIH HHS R15 AI156771NIMHD NIH HHS U54 MD012388NIMHD NIH HHS U54MD012388
6 · The paper itself

Abstract

aimsTo understand the impact of storage temperature on recovery of Staphylococcus aureus on sampling swabs. Staphylococcus aureus is a common cause of skin and soft tissue infections, but also causes a variety of life-threatening diseases. With a large pool of asymptomatic carriers and transmission that can occur even through indirect contact, mitigation efforts have had limited success. Swab sampling, followed by culturing, is a cornerstone of epidemiological studies, however, S. aureus viability on swabs stored at different temperatures has not been characterized. METHODS AND

resultsWe determined survival rates on swabs stored at five different temperatures. Samples stored at -70°C had no decay over time while samples stored at higher temperatures showed an exponential decay in viability. Mortality rates were greatest for swabs stored at 37°C. Survival at intermediate temperatures (-20 to 20·5°C) did not differ significantly, however, we observed more variation at higher temperatures.

conclusionsTo maximize recovery of S. aureus cells, samples should be stored at -70°C or processed for culturing without delay. SIGNIFICANCE AND IMPACT OF THE STUDY: Epidemiological studies of bacterial diseases are typically limited to determination of pathogen presence/absence, yet quantitative assessments of pathogen load and genetic diversity can provide insights into disease progression and severity, likelihood of transmission and adaptive evolutionary potential. For studies of S. aureus where time or access to a microbiology laboratory may delay culturing, deep freezing or timely culturing will maximize the degree to which sampling results reflect source status.

Indexed as

Staphylococcal InfectionsStaphylococcus aureusHot TemperatureHumansSpecimen HandlingTemperaturebacterial storage conditionscommunity sampling of S. aureusdetermining bacterial concentrationmicrobial viabilitystoring bacterial samplessurvival of bacteria on swabsswab samples

Identifiers

PMID33544965
PMCPMC8339145

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.