ArticlePloS one2021
Impact of gestational low-protein intake on embryonic kidney microRNA expression and in nephron progenitor cells of the male fetus.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 18 citations in OpenAlex.
- Maternal protein restriction programs the ageing kidney: Proteomic signatures of early-life origin renal and metabolic dysfunction.The Journal of physiology · 2026Article
- The Impact of Low-Protein Diet on the Molecular and Cellular Development of the Fetal Kidney.Journal of the American Society of Nephrology : JASN · 2026Article
- Effects of gestational protein restriction on autophagy dynamics during odontogenesis.Cell and tissue research · 2026Article
- Renal proteomics of male offspring exposed to maternal protein restriction: molecular, epigenetic, and nephron-specific signatures of metabolic programming.Journal of physiology and biochemistry · 2026Article
- Early and long-term effects of maternal protein restriction on offspring organs and systems: insights from the developmental origins of health and disease (DOHaD).Biogerontology · 2025Review
- The earliest impact of restricted protein intake during pregnancy on heart development in male mouse offspring.Frontiers in cell and developmental biology · 2025Article
- Early metabolic and hemodynamic indicators of kidney dysfunction in mice offspring from parental low protein diet.Molecular and cellular pediatrics · 2024Article
- Mandible development under gestational protein restriction: cellular and molecular mechanisms.Journal of molecular histology · 2024Article
- Impact of maternal protein restriction on Hypoxia-Inducible Factor (HIF) expression in male fetal kidney development.PloS one · 2023Article
- Regulation of nephron progenitor cell lifespan and nephron endowment.Nature reviews. Nephrology · 2022Review
- Article
- Transcriptome and morphological analysis on the heart in gestational protein-restricted aging male rat offspring.Frontiers in cell and developmental biology · 2022Article
- Fetal Undernutrition Programming, Sympathetic Nerve Activity, and Arterial Hypertension Development.Frontiers in physiology · 2021Review
- Gestational Low Protein Diet Modulation on miRNA Transcriptome and Its Target During Fetal and Breastfeeding Nephrogenesis.Frontiers in physiology · 2021Article
- The effects of pregnancy on the progression of maternal glomerular disease.Jornal brasileiro de nefrologiaArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHere, we have demonstrated that gestational low-protein (LP) intake offspring present lower birth weight, reduced nephron numbers, renal salt excretion, arterial hypertension, and renal failure development compared to regular protein (NP) intake rats in adulthood. We evaluated the expression of various miRNAs and predicted target genes in the kidney in gestational 17-days LP (DG-17) fetal metanephros to identify molecular pathways involved in the proliferation and differentiation of renal embryonic or fetal cells.
methodsPregnant Wistar rats were classified into two groups based on protein supply during pregnancy: NP (regular protein diet, 17%) or LP diet (6%). Renal miRNA sequencing (miRNA-Seq) performed on the MiSeq platform, RT-qPCR of predicted target genes, immunohistochemistry, and morphological analysis of 17-DG NP and LP offspring were performed using previously described methods.
resultsA total of 44 miRNAs, of which 19 were up and 25 downregulated, were identified in 17-DG LP fetuses compared to age-matched NP offspring. We selected 7 miRNAs involved in proliferation, differentiation, and cellular apoptosis. Our findings revealed reduced cell number and Six-2 and c-Myc immunoreactivity in metanephros cap (CM) and ureter bud (UB) in 17-DG LP fetuses. Ki-67 immunoreactivity in CM was 48% lesser in LP compared to age-matched NP fetuses. Conversely, in LP CM and UB, β-catenin was 154%, and 85% increased, respectively. Furthermore, mTOR immunoreactivity was higher in LP CM (139%) and UB (104%) compared to that in NP offspring. TGFβ-1 positive cells in the UB increased by approximately 30% in the LP offspring. Moreover, ZEB1 metanephros-stained cells increased by 30% in the LP offspring. ZEB2 immunofluorescence, although present in the entire metanephros, was similar in both experimental groups.
conclusionsMaternal protein restriction changes the expression of miRNAs, mRNAs, and proteins involved in proliferation, differentiation, and apoptosis during renal development. Renal ontogenic dysfunction, caused by maternal protein restriction, promotes reduced reciprocal interaction between CM and UB; consequently, a programmed and expressive decrease in nephron number occurs in the fetus.
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