ArticleAnalytical and bioanalytical chemistry2021
3D-surface MALDI mass spectrometry imaging for visualising plant defensive cardiac glycosides in Asclepias curassavica.
Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 22 citations in OpenAlex.
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- Mass Spectrometry Imaging Combined With Single-Cell Transcriptional Profiling Reveals the Multidimensional Spatial Distributions and Biosynthetic Pathways of Medicinal Components in Andrographis paniculata.Plant biotechnology journal · 2026Article
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- DESI-MSI applications for direct (spatial) biomolecular analysis of South African natural medicinal products.Frontiers in plant science · 2026Article
- Plant metabolism: Zoom in to the single-cell level.Plant physiology · 2025Review
- Improvements in Fast Mass Microscopy for Large-Area Samples.Analytical chemistry · 2024Article
- Applications of mass spectrometry imaging in botanical research.Advanced biotechnology · 2024Review
- Nanoparticle-based applications by atmospheric pressure matrix assisted desorption/ionization mass spectrometry.Nanoscale advances · 2023Review
- Natural or artificial: An example of topographic spatial distribution analysis of mescaline in cactus plants by matrix-assisted laser desorption/ionization mass spectrometry imaging.Frontiers in plant science · 2023Article
- MALDI MS Imaging of Cucumbers.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Infrared Laser Ablation Microsampling with a Reflective Objective.Journal of the American Society for Mass Spectrometry · 2022Article
- Advanced applications of mass spectrometry imaging technology in quality control and safety assessments of traditional Chinese medicines.Journal of ethnopharmacology · 2022Review
- Metabolomics and health: from nutritional crops and plant-based pharmaceuticals to profiling of human biofluids.Cellular and molecular life sciences : CMLS · 2021Review
- Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2021-2022.Mass spectrometry reviewsReview
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Mass spectrometry-based imaging (MSI) has emerged as a promising method for spatial metabolomics in plant science. Several ionisation techniques have shown great potential for the spatially resolved analysis of metabolites in plant tissue. However, limitations in technology and methodology limited the molecular information for irregular 3D surfaces with resolutions on the micrometre scale. Here, we used atmospheric-pressure 3D-surface matrix-assisted laser desorption/ionisation mass spectrometry imaging (3D-surface MALDI MSI) to investigate plant chemical defence at the topographic molecular level for the model system Asclepias curassavica. Upon mechanical damage (simulating herbivore attacks) of native A. curassavica leaves, the surface of the leaves varies up to 700 μm, and cardiac glycosides (cardenolides) and other defence metabolites were exclusively detected in damaged leaf tissue but not in different regions of the same leaf. Our results indicated an increased latex flow rate towards the point of damage leading to an accumulation of defence substances in the affected area. While the concentration of cardiac glycosides showed no differences between 10 and 300 min after wounding, cardiac glycosides decreased after 24 h. The employed autofocusing AP-SMALDI MSI system provides a significant technological advancement for the visualisation of individual molecule species on irregular 3D surfaces such as native plant leaves. Our study demonstrates the enormous potential of this method in the field of plant science including primary metabolism and molecular mechanisms of plant responses to abiotic and biotic stress and symbiotic relationships.
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