Evidence map›Paper›PMID 33544079›Full record

ArticleeLife2021

Membrane-partitioned cell wall synthesis in mycobacteria.

Alam García-Heredia, Takehiro Kado, Caralyn E Sein, Julia Puffal, Sarah H Osman, Julius Judd, Todd A Gray, Yasu S Morita, M Sloan Siegrist

Open access · goldAbstract read
In one paragraph

Article in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed
6.4field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 47 citations in OpenAlex.

  1. Journal of bacteriology · 2026
    Article
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  5. Loss ofbioRxiv : the preprint server for biology · 2025
    Article
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  7. Review
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  10. Chemical biology tools to probe bacterial glycans.Current opinion in chemical biology · 2024
    Review
  11. Article
  12. Bacterial lipid biophysics and membrane organization.Current opinion in microbiology · 2023
    Review
  13. Article
  14. Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Alam García-HerediaMolecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, United States.ORCID 0000-0002-9573-4087
Takehiro KadoDepartment of Microbiology, University of Massachusetts, Amherst, United States.
Caralyn E SeinDepartment of Microbiology, University of Massachusetts, Amherst, United States.
Julia PuffalDepartment of Microbiology, University of Massachusetts, Amherst, United States.ORCID 0000-0003-3066-5225
Sarah H OsmanDepartment of Microbiology, University of Massachusetts, Amherst, United States.
Julius JuddDivision of Genetics, Wadsworth Center, New York State Department of Health, Albany, United States.ORCID 0000-0002-4602-0205
Todd A GrayDivision of Genetics, Wadsworth Center, New York State Department of Health, Albany, United States.
Yasu S MoritaMolecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, United States.ORCID 0000-0002-4514-9242
M Sloan SiegristMolecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, United States.ORCID 0000-0002-8232-3246
University of Massachusetts Amherst · USNew York State Department of Health · US

Funding

A Community Mycobacterial Systems ResourceR01AI097191 · NIAID · WADSWORTH CENTER · PI DERBYSHIRE, KEITH M, FORTUNE, SARAH · 2012 to 2016
$3.2M
Chemical detection of Mycobacterium tuberculosis growth and antibiotic response during infectionDP2AI138238 · NIAID · UNIVERSITY OF MASSACHUSETTS AMHERST · PI SIEGRIST, MARY SLOAN · 2017 to 2017
$2.3M
Metabolic carbohydrate cell wall probes for bacterial structure and immune recognition studiesU01CA221230 · NCI · UNIVERSITY OF DELAWARE · PI GRIMES, CATHERINE LEIMKUHLER · 2017 to 2019
$1.1M
Spatial organization of mycobacterial cell wall biosynthesisR21AI144748 · NIAID · UNIVERSITY OF MASSACHUSETTS AMHERST · PI MORITA, YASU S, SIEGRIST, MARY SLOAN · 2019 to 2020
$423k
Membrane domain dynamics in Mycobacterium tuberculosisR03AI140259 · NIAID · UNIVERSITY OF MASSACHUSETTS AMHERST · PI MORITA, YASU S · 2018 to 2019
$160k
NCI NIH HHS U01 CA221230NIAID NIH HHS DP2 AI138238NIAID NIH HHS R01 AI097191NIAID NIH HHS R03 AI140259NIAID NIH HHS R21 AI144748NIH HHS R21 AI144748
6 · The paper itself

Abstract

Many antibiotics target the assembly of cell wall peptidoglycan, an essential, heteropolymeric mesh that encases most bacteria. In rod-shaped bacteria, cell wall elongation is spatially precise yet relies on limited pools of lipid-linked precursors that generate and are attracted to membrane disorder. By tracking enzymes, substrates, and products of peptidoglycan biosynthesis in

Indexed as

Cell CycleCell MembraneCell WallMycobacterium smegmatisPeptidoglycanPeptidoglycanbiochemistrycell wallchemical biologymembrane domainsmetabolic labelingmicrobiology and infectious diseaseM. smegmatispeptidoglycan

Identifiers

PMID33544079
PMCPMC7864634
OpenAlexW3127218429

What OpenQuestion holds

Textmetadata
LicenceCC0
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.